基于电酶组装策略的代谢产物高效同时检测。

IF 5 Q1 ENGINEERING, BIOMEDICAL
BME frontiers Pub Date : 2023-09-19 eCollection Date: 2023-01-01 DOI:10.34133/bmef.0027
Anran Zheng, Chao Li, Shengkai Xu, Zhen Guo, Chuanyu Li, Changsong Zhang, Jia Yao, Zhiqi Zhang, Jinze Li, Lutao Du, Shasha Zhao, Chuanxin Wang, Wei Zhang, Lianqun Zhou
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引用次数: 0

摘要

目标和影响声明:我们描述了一种基于电酶组装峰分离策略的电酶介体(EM)传感器,该传感器可以在一次测试中有效地同时检测5μl血浆中的3种典型心血管疾病(CVD)代谢产物。这项工作对提高慢性CVD评估的效率具有重要意义。引言:监测代谢产物的心血管疾病与疾病风险密切相关。在医院进行独立且耗时的检测不利于慢性CVD的管理。方法:通过电子介质和酶的交联,灵活设计EM,并在一个电极上组装3层不同特性的EM层。电子在可调电位下转移;通过与代谢物浓度相关的3个峰值电流定量检测3种代谢物。结果:在本研究中,EM传感器对3种代谢产物的同时检测显示出高灵敏度,下限为0.01mM。242名患者的传感器与临床的线性相关性大于0.980,风险评估的一致性为94.6%。结论:EM可以扩展代谢产物,并且该传感器可能是用于CVD风险评估的家庭医疗保健工具的有前途的候选者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Efficient Simultaneous Detection of Metabolites Based on Electroenzymatic Assembly Strategy.

Efficient Simultaneous Detection of Metabolites Based on Electroenzymatic Assembly Strategy.

Efficient Simultaneous Detection of Metabolites Based on Electroenzymatic Assembly Strategy.

Efficient Simultaneous Detection of Metabolites Based on Electroenzymatic Assembly Strategy.

Objective and Impact Statement: We describe an electroenzymatic mediator (EM) sensor based on an electroenzymatic assembly peak separation strategy, which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease (CVD) metabolites in 5 μl of plasma under one test. This work has substantial implications toward improving the efficiency of chronic CVD assessment. Introduction: Monitoring CVD of metabolites is strongly associated with disease risk. Independent and time-consuming detection in hospitals is unfavorable for chronic CVD management. Methods: The EM was flexibly designed by the cross-linking of electron mediators and enzymes, and 3 EM layers with different characteristics were assembled on one electrode. Electrons were transferred under tunable potential; 3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite concentrations. Results: In this study, the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 mM. The linear correlation between the sensor and clinical was greater than 0.980 for 242 patients, and the consistency of risk assessment was 94.6%. Conclusion: Metabolites could be expanded by the EM, and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.

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CiteScore
7.10
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审稿时长
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