Ana S. Ramírez , José B. Poveda , Remco Dijkman , Carlos Poveda , Alejandro Suárez-Pérez , Rubén S. Rosales , Anneke Feberwee , Michael P. Szostak , Lorenzo Ressel , Tomeu Viver , Pascual Calabuig , Salvatore Catania , Federica Gobbo , Dorina Timofte , Joachim Spergser
{"title":"从海鸟气管中分离的bradburyae支原体。","authors":"Ana S. Ramírez , José B. Poveda , Remco Dijkman , Carlos Poveda , Alejandro Suárez-Pérez , Rubén S. Rosales , Anneke Feberwee , Michael P. Szostak , Lorenzo Ressel , Tomeu Viver , Pascual Calabuig , Salvatore Catania , Federica Gobbo , Dorina Timofte , Joachim Spergser","doi":"10.1016/j.syapm.2023.126472","DOIUrl":null,"url":null,"abstract":"<div><p><span>In the search for mollicutes in wild birds, six </span><span><em>Mycoplasma</em></span> strains were isolated from tracheal swabs taken from four different species of seabirds. Four strains originated from three Yellow-legged gulls (<em>Larus michahellis</em>) and a Cory’s shearwater (<span><em>Calonectris</em><em> borealis</em></span>) from Spain<em>,</em><span> one from a South African Kelp gull (</span><em>Larus dominicanus</em>)<em>,</em> and one from an Italian Black-headed gull (<span><em>Chroicocephalus</em><em> ridibundus</em></span>). These <em>Mycoplasma</em><span> strains presented 99 % 16S rRNA gene sequence similarity values with </span><em>Mycoplasma</em> (<em>M.</em>) <em>gallisepticum</em><span>. Phylogenetic analyses of marker genes (16S rRNA gene and </span><em>rpoB</em>) confirmed the close relationship of the strains to <em>M. gallisepticum</em> and <em>M. tullyi</em><span><span><span><span><span>. The seabirds’ strains grew well in modified Hayflick medium, and colonies showed typical fried egg morphology. They produced acid from glucose and mannose but did not </span>hydrolyze arginine or urea. </span>Transmission electron microscopy revealed a cell morphology characteristic of mycoplasmas, presenting spherical to flask-shaped cells with an attachment </span>organelle. </span>Gliding motility<span> was also observed. Furthermore, serological tests, MALDI-ToF mass spectrometry and genomic studies demonstrated that the strains were different to any known </span></span><em>Mycoplasma</em> species, for which the name <em>Mycoplasma bradburyae</em> sp. nov. is proposed; the type strain is T158<sup>T</sup> (DSM 110708 = NCTC 14398).</p></div>","PeriodicalId":22124,"journal":{"name":"Systematic and applied microbiology","volume":"46 6","pages":"Article 126472"},"PeriodicalIF":3.3000,"publicationDate":"2023-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mycoplasma bradburyae sp. nov. isolated from the trachea of sea birds\",\"authors\":\"Ana S. Ramírez , José B. Poveda , Remco Dijkman , Carlos Poveda , Alejandro Suárez-Pérez , Rubén S. Rosales , Anneke Feberwee , Michael P. Szostak , Lorenzo Ressel , Tomeu Viver , Pascual Calabuig , Salvatore Catania , Federica Gobbo , Dorina Timofte , Joachim Spergser\",\"doi\":\"10.1016/j.syapm.2023.126472\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>In the search for mollicutes in wild birds, six </span><span><em>Mycoplasma</em></span> strains were isolated from tracheal swabs taken from four different species of seabirds. Four strains originated from three Yellow-legged gulls (<em>Larus michahellis</em>) and a Cory’s shearwater (<span><em>Calonectris</em><em> borealis</em></span>) from Spain<em>,</em><span> one from a South African Kelp gull (</span><em>Larus dominicanus</em>)<em>,</em> and one from an Italian Black-headed gull (<span><em>Chroicocephalus</em><em> ridibundus</em></span>). These <em>Mycoplasma</em><span> strains presented 99 % 16S rRNA gene sequence similarity values with </span><em>Mycoplasma</em> (<em>M.</em>) <em>gallisepticum</em><span>. Phylogenetic analyses of marker genes (16S rRNA gene and </span><em>rpoB</em>) confirmed the close relationship of the strains to <em>M. gallisepticum</em> and <em>M. tullyi</em><span><span><span><span><span>. The seabirds’ strains grew well in modified Hayflick medium, and colonies showed typical fried egg morphology. They produced acid from glucose and mannose but did not </span>hydrolyze arginine or urea. </span>Transmission electron microscopy revealed a cell morphology characteristic of mycoplasmas, presenting spherical to flask-shaped cells with an attachment </span>organelle. </span>Gliding motility<span> was also observed. Furthermore, serological tests, MALDI-ToF mass spectrometry and genomic studies demonstrated that the strains were different to any known </span></span><em>Mycoplasma</em> species, for which the name <em>Mycoplasma bradburyae</em> sp. nov. is proposed; the type strain is T158<sup>T</sup> (DSM 110708 = NCTC 14398).</p></div>\",\"PeriodicalId\":22124,\"journal\":{\"name\":\"Systematic and applied microbiology\",\"volume\":\"46 6\",\"pages\":\"Article 126472\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2023-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Systematic and applied microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0723202023000814\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic and applied microbiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0723202023000814","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Mycoplasma bradburyae sp. nov. isolated from the trachea of sea birds
In the search for mollicutes in wild birds, six Mycoplasma strains were isolated from tracheal swabs taken from four different species of seabirds. Four strains originated from three Yellow-legged gulls (Larus michahellis) and a Cory’s shearwater (Calonectris borealis) from Spain, one from a South African Kelp gull (Larus dominicanus), and one from an Italian Black-headed gull (Chroicocephalus ridibundus). These Mycoplasma strains presented 99 % 16S rRNA gene sequence similarity values with Mycoplasma (M.) gallisepticum. Phylogenetic analyses of marker genes (16S rRNA gene and rpoB) confirmed the close relationship of the strains to M. gallisepticum and M. tullyi. The seabirds’ strains grew well in modified Hayflick medium, and colonies showed typical fried egg morphology. They produced acid from glucose and mannose but did not hydrolyze arginine or urea. Transmission electron microscopy revealed a cell morphology characteristic of mycoplasmas, presenting spherical to flask-shaped cells with an attachment organelle. Gliding motility was also observed. Furthermore, serological tests, MALDI-ToF mass spectrometry and genomic studies demonstrated that the strains were different to any known Mycoplasma species, for which the name Mycoplasma bradburyae sp. nov. is proposed; the type strain is T158T (DSM 110708 = NCTC 14398).
期刊介绍:
Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology: