从海鸟气管中分离的bradburyae支原体。

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Ana S. Ramírez , José B. Poveda , Remco Dijkman , Carlos Poveda , Alejandro Suárez-Pérez , Rubén S. Rosales , Anneke Feberwee , Michael P. Szostak , Lorenzo Ressel , Tomeu Viver , Pascual Calabuig , Salvatore Catania , Federica Gobbo , Dorina Timofte , Joachim Spergser
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引用次数: 0

摘要

为了在野生鸟类中寻找毛霉菌门,从四种不同海鸟的气管拭子中分离出六种支原体菌株。四个品系起源于三只来自西班牙的黄腿海鸥(Larus michahellis)和一只科里剪尾海鸥(Calonectris borealis),一只来自南非的海带海鸥(Lars dominicanus),另一只来自意大利的黑头海鸥(Chroicocephalus ridibundus)。这些支原体菌株与鸡毒支原体16S rRNA基因序列具有99%的相似性。标记基因(16S rRNA基因和rpoB)的系统发育分析证实了菌株与鸡败血病分枝杆菌和tullyi分枝杆菌的密切关系。海鸟的品系在改良的Hayflick培养基中生长良好,菌落表现出典型的煎蛋形态。它们从葡萄糖和甘露糖中产生酸,但不水解精氨酸或尿素。透射电子显微镜显示支原体的细胞形态特征,呈现球形至烧瓶状细胞,具有附着细胞器。还观察到滑翔运动。此外,血清学测试、MALDI-ToF质谱和基因组研究表明,这些菌株与任何已知的支原体物种都不同,因此提出了bradburyae sp.nov.支原体的名称;类型菌株为T158T(DSM 110708=NCTC 14398)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mycoplasma bradburyae sp. nov. isolated from the trachea of sea birds

In the search for mollicutes in wild birds, six Mycoplasma strains were isolated from tracheal swabs taken from four different species of seabirds. Four strains originated from three Yellow-legged gulls (Larus michahellis) and a Cory’s shearwater (Calonectris borealis) from Spain, one from a South African Kelp gull (Larus dominicanus), and one from an Italian Black-headed gull (Chroicocephalus ridibundus). These Mycoplasma strains presented 99 % 16S rRNA gene sequence similarity values with Mycoplasma (M.) gallisepticum. Phylogenetic analyses of marker genes (16S rRNA gene and rpoB) confirmed the close relationship of the strains to M. gallisepticum and M. tullyi. The seabirds’ strains grew well in modified Hayflick medium, and colonies showed typical fried egg morphology. They produced acid from glucose and mannose but did not hydrolyze arginine or urea. Transmission electron microscopy revealed a cell morphology characteristic of mycoplasmas, presenting spherical to flask-shaped cells with an attachment organelle. Gliding motility was also observed. Furthermore, serological tests, MALDI-ToF mass spectrometry and genomic studies demonstrated that the strains were different to any known Mycoplasma species, for which the name Mycoplasma bradburyae sp. nov. is proposed; the type strain is T158T (DSM 110708 = NCTC 14398).

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CiteScore
7.20
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4.30%
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