遗传密码扩展技术在真核生物中的应用。

IF 13.6 1区生物学 Q1 CELL BIOLOGY

Protein & Cell Pub Date : 2024-05-07 DOI:10.1093/procel/pwad051

Qiao-Ru Guo, Yu J Cao

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引用次数: 0

摘要

非天然氨基酸（UAAs）由于能够为蛋白质引入新的化学功能，在蛋白质工程和药物开发中受到了极大的关注。在真核生物中，遗传密码扩展（GCE）能够掺入UAA并促进转录后修饰（PTM），这在原核系统中是不可行的。GCE也是细胞或动物成像、监测靶细胞中蛋白质相互作用、药物开发和开关调节的强大工具。因此，人们对在真核系统中利用GCE产生了浓厚的兴趣。这篇综述概述了GCE在真核系统中的应用，并讨论了当前需要解决的挑战。

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Applications of genetic code expansion technology in eukaryotes.

Unnatural amino acids (UAAs) have gained significant attention in protein engineering and drug development owing to their ability to introduce new chemical functionalities to proteins. In eukaryotes, genetic code expansion (GCE) enables the incorporation of UAAs and facilitates posttranscriptional modification (PTM), which is not feasible in prokaryotic systems. GCE is also a powerful tool for cell or animal imaging, the monitoring of protein interactions in target cells, drug development, and switch regulation. Therefore, there is keen interest in utilizing GCE in eukaryotic systems. This review provides an overview of the application of GCE in eukaryotic systems and discusses current challenges that need to be addressed.

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来源期刊

Protein & Cell CELL BIOLOGY-

CiteScore

24.00

自引率

0.90%

发文量

1029

审稿时长

6-12 weeks

期刊介绍： Protein & Cell is a monthly, peer-reviewed, open-access journal focusing on multidisciplinary aspects of biology and biomedicine, with a primary emphasis on protein and cell research. It publishes original research articles, reviews, and commentaries across various fields including biochemistry, biophysics, cell biology, genetics, immunology, microbiology, molecular biology, neuroscience, oncology, protein science, structural biology, and translational medicine. The journal also features content on research policies, funding trends in China, and serves as a platform for academic exchange among life science researchers.