汉森尼德拜菌的开放(非无菌)培养用于重组蛋白生产,结合高盐含量的工业支流。

IF 4.5 2区 生物学 Q1 BIOCHEMICAL RESEARCH METHODS
Mònica Estrada , Clara Navarrete , Sønke Møller , Manuel Quirós , José L. Martínez
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引用次数: 0

摘要

耐盐的非传统酵母汉氏Debaryomyces hansenii可以在含有高浓度盐(高达4M)的培养基中生长,代谢葡萄糖以外的替代碳源,如乳糖或甘油,并能承受广泛的温度和pH。这些固有的能力使这种酵母能够在恶劣的环境中生长,并使用传统商业培养基以外的替代原料。例如,D.hansenii可能是一家潜在的细胞工厂,用于重新评估工业含盐副产品的价值,将其用作生产新的有价值生物产品的基质,促进循环经济。在这项工作中,来自乳制品和生物制药行业的三种不同的含盐副产品被测试为D.hansenii生长的可能原料。酵母不仅能够在所有酵母中有效生长,而且能够在不改变其性能的情况下产生重组蛋白(黄色荧光蛋白,用作模型)。此外,在非无菌条件下进行不同实验室规模(1.5mL和1L)的开放培养,不向培养中添加淡水或任何营养补充剂,使该过程更便宜、更可持续。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content

Open (non-sterile) cultivations of Debaryomyces hansenii for recombinant protein production combining industrial side-streams with high salt content

The halotolerant non-conventional yeast Debaryomyces hansenii can grow in media containing high concentrations of salt (up to 4 M), metabolize alternative carbon sources than glucose, such as lactose or glycerol, and withstand a wide range of temperatures and pH. These inherent capabilities allow this yeast to grow in harsh environments and use alternative feedstock than traditional commercial media. For example, D. hansenii could be a potential cell factory for revalorizing industrial salty by-products, using them as a substrate for producing new valuable bioproducts, boosting a circular economy. In this work, three different salty by-products derived from the dairy and biopharmaceutical industry have been tested as a possible feedstock for D. hansenii’s growth. The yeast was not only able to grow efficiently in all of them but also to produce a recombinant protein (Yellow Fluorescent Protein, used as a model) without altering its performance. Moreover, open cultivations at different laboratory scales (1.5 mL and 1 L) were performed under non-sterile conditions and without adding fresh water or any nutritional supplement to the cultivation, making the process cheaper and more sustainable.

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来源期刊
New biotechnology
New biotechnology 生物-生化研究方法
CiteScore
11.40
自引率
1.90%
发文量
77
审稿时长
1 months
期刊介绍: New Biotechnology is the official journal of the European Federation of Biotechnology (EFB) and is published bimonthly. It covers both the science of biotechnology and its surrounding political, business and financial milieu. The journal publishes peer-reviewed basic research papers, authoritative reviews, feature articles and opinions in all areas of biotechnology. It reflects the full diversity of current biotechnology science, particularly those advances in research and practice that open opportunities for exploitation of knowledge, commercially or otherwise, together with news, discussion and comment on broader issues of general interest and concern. The outlook is fully international. The scope of the journal includes the research, industrial and commercial aspects of biotechnology, in areas such as: Healthcare and Pharmaceuticals; Food and Agriculture; Biofuels; Genetic Engineering and Molecular Biology; Genomics and Synthetic Biology; Nanotechnology; Environment and Biodiversity; Biocatalysis; Bioremediation; Process engineering.
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