新疆白僵菌MJ1015的生物学特性及固体培养基产孢工艺的优化。

Polish journal of microbiology Pub Date : 2023-12-16 eCollection Date: 2023-12-01 DOI:10.33073/pjm-2023-033
Xinghong Wang, Zhenxing Huang, Congchun Li, Man Liu, Guangming Yang, Lilin Luo, Yongchao Rao, Yan Shen, Jing Wang
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引用次数: 0

摘要

从贵州某蓝莓农场的幼虫中分离得到的昆虫病原真菌马江根白僵菌MJ1015菌株可作为生物防治剂。作为确定不同固体培养基、温度和pH对菌落生长速率和孢子形成的影响的第一步,我们在商业规模上评估了菌丝生长和分生孢子的最佳固体培养基。随后,我们还使用单因素分析和响应面优化来优化固体培养基的组成。在马铃薯葡萄糖琼脂(PDA)培养基上,MJ1015在29°C和pH值为5时生长最快,产孢量最高。菌株MJ1015生长和产孢的最佳固体培养基包括64.70g/l的水稻、13.00g/l的小麦、0.30g/l的NaNO3、0.36g/l的K2HPO4·3H2O和1.00g/l的CaCO3。水稻、NaNO3和K2HPO4·3H2O是主要影响因素。用最佳培养基培养孢子的预测值为4.56×1010分生孢子/l。验证试验结果表明,菌株MJ1015在最佳培养基上的平均生长速率分别比在沙氏葡萄糖琼脂酵母提取物培养基(SDAY)和PDA上的平均增长速率快85%和96%。孢子产生量分别是SDAY和PDA的43.90倍和9.65倍。我们的研究结果为商业化生产马江根B.majiangensis以控制幼虫提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biological Characteristics of Beauveria majiangensis Strain MJ1015 and Optimization of Solid Medium Technology for Sporulation.

The entomopathogenic fungus Beauveria majiangensis strain MJ1015, recently isolated from white grubs on a blueberry farm in Guizhou, China, could be used as a biocontrol agent. As a first step toward determining the effect of different solid culture media, temperature, and pH on colony growth rate and sporulation, we evaluated the optimum solid medium for mycelial growth and conidia production on a commercial scale. Subsequently, we also used single-factor analysis and response surface optimization to optimize the composition of the solid culture medium. On potato dextrose agar (PDA) medium, MJ1015 grew fastest and produced the highest spore yield at 29°C and pH 5. The best solid medium for the growth and sporulation of strain MJ1015 comprised 64.70 g/l of rice, 13.00 g/l of wheat, 0.30 g/l of NaNO3, 0.36 g/l of K2HPO4 · 3H2O, and 1.00 g/l of CaCO3. Rice, NaNO3, and K2HPO4 · 3H2O were the main influencing factors. The predicted value of cultured spores using the optimal medium was 4.56 x 1010 conidia/l. The validation test results showed that the average growth rate of strain MJ1015 on the optimal medium was 85% and 96% faster than that on Sabouraud dextrose agar with yeast extracts medium (SDAY) and PDA, respectively. Sporulation was 43.90 times and 9.65 times of that produced on SDAY and PDA, respectively. Our findings provide a theoretical basis for the commercial production of B. majiangensis to control white grubs.

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