神经激肽-1受体敲除对变应性鼻炎RANTES表达的影响。

IF 2.5 3区 医学 Q1 OTORHINOLARYNGOLOGY
American Journal of Rhinology & Allergy Pub Date : 2023-11-01 Epub Date: 2023-07-31 DOI:10.1177/19458924231191012
Hong Wang, Jing Wu, Ruxin Zhang
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引用次数: 1

摘要

背景:神经激肽-1受体(NK-1R)和正常T细胞表达和分泌(RANTES)在变应性鼻炎(AR)中起着重要作用。然而,NK-1R在AR中的调节作用是否通过RANTES实现仍然未知。方法:采用卵清蛋白致敏激发SD大鼠,建立AR模型。在激发期,NKR组用NK-1R特异性小干扰RNA(siRNA)鼻内治疗大鼠,NCS组用阴性siRNA鼻内治疗,NSAR组和NS组给大鼠生理盐水。测量每只大鼠的鼻腔分泌物量以及擦鼻和打喷嚏的次数。通过实时荧光定量RT-PCR和免疫组织化学染色测定鼻粘膜组织中NK-1R和RANTES的水平。计数收集的鼻腔灌洗液(NLF)中嗜酸性粒细胞的数量,并通过酶联免疫吸附法测定NLF中RANTES的浓度。结果:与NS组相比,NSAR组和NCS组大鼠鼻粘膜NK-1R和RANTES的表达显著高于NS组。NSAR组和NCS组的喷嚏、擦鼻次数和鼻腔分泌物量显著增加。NKR组大鼠的AR症状比NSAR和NCS组大鼠得到更大的缓解。此外,NK-1R表达的敲低也显著消除了NKR组大鼠鼻粘膜中RANTES的表达和嗜酸性粒细胞的浸润。结论:我们首次发现,用NK-1R特异性siRNA鼻内治疗可以显著降低RANTES的表达、AR相关症状和嗜酸性粒细胞炎症,这表明NK-1R在AR发展中的调节作用是通过改变RANTES表达来实现的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of Neurokinin-1 Receptor Knockdown on the Expression of RANTES in Allergic Rhinitis.

Background: Neurokinin-1 receptor (NK-1R) and normal T cell expressed and secreted (RANTES) have been shown to play important roles in allergic rhinitis (AR). However, whether the regulating effect of NK-1R in AR is achieved via RANTES remains unknown.

Methods: In the present study, Sprague-Dawley rats were sensitized and challenged with ovalbumin to make AR models. During the challenge period, the rats were treated intranasally with NK-1R-specific small interfering RNA (siRNA) for NKR group, negative siRNA for NCS group, rats in NSAR group and NS group were given saline. The amount of nasal secretion and the numbers of nose rubs and sneezes were measured in each rat. The levels of NK-1R and RANTES in the nasal mucosal tissues were determined through real-time fluorescence quantitative RT-PCR and immunohistochemical staining. The numbers of eosinophils in the collected nasal lavage fluid (NLF) were counted, and the concentration of RANTES in NLF was determined by enzyme-linked immunosorbent assay.

Results: Compared with that in the NS group, the expression of NK-1R and RANTES was significantly higher in the nasal mucosa of NSAR and NCS group rats. The sneezing and nose rubbing counts and the amount of nasal secretions were increased significantly in the NSAR and NCS groups. Rats in the NKR group experienced greater relief from AR symptoms than rats in the NSAR and NCS groups. Furthermore, knockdown of NK-1R expression also significantly eliminated RANTES expression and eosinophil infiltration in the nasal mucosa of NKR group rats.

Conculsion: For the first time, we show that intranasal treatment with NK-1R-specific siRNA can significantly decrease RANTES expression, AR-related symptoms, and eosinophil inflammation, suggesting that the regulating effect of NK-1R in the development of AR occurs via alteration of RANTES expression.

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
82
审稿时长
4-8 weeks
期刊介绍: The American Journal of Rhinology & Allergy is a peer-reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials, and review articles.
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