DnaK和SpaA重组蛋白在小鼠模型中对赤藓病的免疫原性和保护作用。

IF 2.5 Q3 CELL BIOLOGY
Naiane Lima Godoy, Jonathan Ballico de Moraes, Cynthia Aparecida de Castro, Jhonne Pedro Pedott Santana, Teresa Cristina Zangirolami, Adilson José da Silva, Maria Teresa Marques Novo-Mansur, Fernanda de Freitas Anibal
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引用次数: 0

摘要

背景/目的:猪丹毒是由一种革兰氏阳性杆菌——猪赤藓引起的一种疾病,由于它会导致猪群的损失,因此具有重要的经济意义。为了控制这种疾病,用杀死或减毒的蛇颈杆菌细胞疫苗对动物进行免疫,但即使进行群体疫苗接种,美国、中国和日本也报告了猪丹毒爆发的病例,这导致了对该细菌的其他抗原成分的寻找,这些成分可能会促进对蛇颈杆菌的更大保护。已经证明,来自蛇颈杆菌的表面蛋白SpaA是构成亚单位疫苗的候选蛋白,因为已经有报道称它可以诱导宿主对该细菌的免疫反应。DnaK,一种hsp70分子伴侣,似乎也是疫苗组成中的一个很好的候选者,因为它已被证明是细菌的抗原蛋白。方法:本工作评估了E.rhusopathiee-SPA和DnaK重组蛋白在小鼠模型中诱导的免疫原性和保护作用,方法是在21天间隔两次给药100µg。与商业疫苗和非疫苗接种条件相比,对候选蛋白进行单独或一起测试,并用蛇颈杆菌的毒力菌株攻击小鼠。收集血清以评估产生的抗体和外周血细胞,而通过菌落计数来检测脾脏和肾脏组织中是否存在蛇颈杆菌。结果:绘制了动物的存活曲线,证实了蛋白质的保护作用。与对照组相比,接种蛋白质的动物血清中的IgG抗体增加,并且观察到疾病症状的显著延迟。结论:上述结果表明,蛇颈草杆菌DnaK和SpaA在小鼠体内具有免疫原性,并干扰疾病的发展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Immunogenicity and Protection by DnaK and SpaA Recombinant Proteins Against Erysipelothrix Rhusiopathiae in a Murine Model.

Background/aims: Swine erysipelas is a disease caused by Erysipelothrix rhusiopathiae, a Gram-positive bacillus, which has great economic importance because it leads to the loss of the swine herd. To control this disease, animals are immunized with a cellular vaccine of killed or attenuated E. rhusiopathiae, but even with herd vaccination, cases of swine erysipelas outbreaks have been reported in the United States, China and Japan, leading to the search for other antigenic components of the bacteria that may promote greater protection against E. rhusiopathiae. The surface protein SpaA from E. rhusiopathiae has been shown to be a candidate to constitute a subunit vaccine, since it has already been reported to induce a host immune response against the bacterium. DnaK, a hsp70 molecular chaperone, also seems to be a good candidate in the composition of a vaccine, as it has been demonstrated to be an antigenic protein of the bacteria.

Methods: This work evaluated the immunogenicity and protection induced by the E. rhusiopathiaee SpaA and DnaK recombinant proteins in a murine model, by intramuscular administration to mice with two doses of 100 µg at 21-day interval between them. The candidate proteins were tested either separately and together, compared with the commercial vaccine and the non-vaccination condition, and mice were challenged with a virulent strain of E. rhusiopathiae. Serum was collected to assess the produced antibodies and peripheral blood cells, whereas spleen and kidney tissues were assayed for E. rhusiopathiae presence by colony counting.

Results: A survival curve of the animals was performed, which confirmed the protection induced by the proteins. IgG antibodies increased in the animal serum inoculated with the proteins when compared to the control, and a significant delay in disease symptoms was observed.

Conclusion: These results suggest that E. rhusiopathiae DnaK and SpaA are immunogenic in mice and interfere with the disease development.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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