支气管镜标本的单细胞RNA测序:快速最小处理方案的开发。

IF 2.2 4区 工程技术 Q3 BIOCHEMICAL RESEARCH METHODS
BioTechniques Pub Date : 2023-10-01 Epub Date: 2023-10-10 DOI:10.2144/btn-2023-0017
Firoozeh V Gerayeli, Stephen Milne, Chen Xi Yang, Xuan Li, Elizabeth Guinto, Julia Shun-Wei Yang, Chung Yan Cheung, Tara R Stach, Tawimas Shaipanich, Janice M Leung, Don D Sin
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引用次数: 0

摘要

单细胞RNA测序(scRNA-seq)是理解包括气道疾病在内的疾病病理生理学的重要工具。目前,大多数气道疾病的scRNA-seq研究都使用了侵入性方法(气道活检、手术切除),这些方法具有固有的风险,因此对气道病理生物学的scRNA-seq研究造成了重大限制。支气管刷毛是一种可行的、侵入性较小的方法,可以获得scRNA-seq的气道细胞。在这里,我们描述了从支气管刷标本中制备scRNA-seq单细胞悬浮液的快速和最小处理方案的开发。我们的优化方案最大限度地提高了细胞回收率和细胞质量,并有助于以单细胞分辨率对气道转录组进行大规模分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Single-cell RNA sequencing of bronchoscopy specimens: development of a rapid minimal handling protocol.

Single-cell RNA sequencing (scRNA-seq) is an important tool for understanding disease pathophysiology, including airway diseases. Currently, the majority of scRNA-seq studies in airway diseases have used invasive methods (airway biopsy, surgical resection), which carry inherent risks and thus present a major limitation to scRNA-seq investigation of airway pathobiology. Bronchial brushing, where the airway mucosa is sampled using a cytological brush, is a viable, less invasive method of obtaining airway cells for scRNA-seq. Here we describe the development of a rapid and minimal handling protocol for preparing single-cell suspensions from bronchial brush specimens for scRNA-seq. Our optimized protocol maximizes cell recovery and cell quality and facilitates large-scale profiling of the airway transcriptome at single-cell resolution.

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来源期刊
BioTechniques
BioTechniques 工程技术-生化研究方法
CiteScore
4.40
自引率
0.00%
发文量
68
审稿时长
3.3 months
期刊介绍: BioTechniques is a peer-reviewed, open-access journal dedicated to publishing original laboratory methods, related technical and software tools, and methods-oriented review articles that are of broad interest to professional life scientists, as well as to scientists from other disciplines (e.g., chemistry, physics, computer science, plant and agricultural science and climate science) interested in life science applications for their technologies. Since 1983, BioTechniques has been a leading peer-reviewed journal for methods-related research. The journal considers: Reports describing innovative new methods, platforms and software, substantive modifications to existing methods, or innovative applications of existing methods, techniques & tools to new models or scientific questions Descriptions of technical tools that facilitate the design or performance of experiments or data analysis, such as software and simple laboratory devices Surveys of technical approaches related to broad fields of research Reviews discussing advancements in techniques and methods related to broad fields of research Letters to the Editor and Expert Opinions highlighting interesting observations or cautionary tales concerning experimental design, methodology or analysis.
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