使用实验性败血症模型比较槲皮素和抗坏血酸对幼年大鼠炎性细胞因子和抗氧化生物标志物的影响。

IF 1.5 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Emine Ufuk Bozkurt, Abdulrahman Ozel, Meltem Erol, Aslihan Tenekecigil, Ozlem Bostan Gayret, Ovgü Buke, Volkan Tosun
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引用次数: 0

摘要

目的:目前正在研究促进抗氧化和抗炎机制的治疗方法,以降低败血症的死亡率。在本研究中,我们使用婴儿大鼠败血症模型比较了槲皮素和抗坏血酸的抗炎和抗氧化活性。方法:将28只21天大的哺乳期刚刚结束的幼鼠分为四组:对照组、败血症组、败血症+槲皮素组和败血症+抗坏血酸组。通过腹膜内注射细菌脂多糖建立败血症模型。24小时后,采集血样,分析血清炎性细胞因子(IL-1β、IL-6、TNF-α和CRP)和抗氧化剂(CAT、GPx、SOD和GST)的水平。结果:败血症+抗坏血酸组的超氧化物歧化酶水平显著高于败血症和败血症+槲皮素组。在接受槲皮素和抗坏血酸治疗的败血症受试者的血清样本中,败血症中最活跃的细胞因子水平显著降低。结论:两种分子均能提高脓毒症患者的抗氧化活性。我们观察到,这两种分子没有副作用,对儿童败血症向严重和致命败血症的进展有积极影响(表2,参考文献38)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of effects of quercetin and ascorbic acid on inflammatory cytokines and antioxidant biomarkers in infant rats using an experimental sepsis model.

Objective: There is ongoing research on treatments that promote antioxidant and anti-inflammatory mechanisms, which will reduce mortality in sepsis. In this study, we compared the anti-inflammatory and antioxidant activities of quercetin and ascorbic acid using a sepsis model induced in infant rats.

Methods: A total of 28 infant rats 21-days-old that had just completed the lactation period were divided into four groups: control, sepsis, sepsis + quercetin, and sepsis + ascorbic acid. The sepsis model was created with an intraperitoneal injection of bacterial lipopolysaccharide. After 24 hours, blood samples were collected for analysis of serum levels of inflammatory cytokines (IL-1β, IL-6, TNF-α, and CRP) and antioxidants (CAT, GPx, SOD, and GST).

Results: The superoxide dismutase levels were significantly higher in the sepsis + ascorbic acid group compared to the sepsis and sepsis + quercetin groups. The levels of the most active cytokines in sepsis were significantly lower in the serum samples of the septic subjects who received quercetin and ascorbic acid.

Conclusion: The antioxidant activity, which is impaired in sepsis, was increased by both molecules. We observed that these two molecules, which are free of side effects, have a positive influence on the progression of sepsis to severe and fatal sepsis in childhood (Tab. 2, Ref. 38).

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来源期刊
CiteScore
2.60
自引率
0.00%
发文量
185
审稿时长
3-8 weeks
期刊介绍: The international biomedical journal - Bratislava Medical Journal – Bratislavske lekarske listy (Bratisl Lek Listy/Bratisl Med J) publishes peer-reviewed articles on all aspects of biomedical sciences, including experimental investigations with clear clinical relevance, original clinical studies and review articles.
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