生产含有脱细胞脊髓组织的生物墨水,用于3D生物打印。

IF 3.5 3区 医学 Q3 CELL & TISSUE ENGINEERING
Tissue Engineering Part A Pub Date : 2024-01-01 Epub Date: 2023-11-15 DOI:10.1089/ten.TEA.2023.0078
Marcelo Garrido Dos Santos, Fernanda Stapenhorst França, João Pedro Prestes, Cristian Teixeira, Luiz Carlos Sommer, Laura Elena Sperling, Patricia Pranke
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引用次数: 0

摘要

在过去的几年里,3D生物打印已经成为再生医学领域一种很有前途的方法。由于其模拟细胞外环境的能力,该技术允许生产支持细胞移植的三维支架。增强细胞粘附、存活和增殖的一种替代方案是使用脱细胞的细胞外基质作为生物墨水成分。本研究的目的是使用冻干大鼠脱细胞脊髓组织(DSCT)生产用于神经组织3D生物打印的生物墨水。DNA定量、苏木精和伊红以及DAPI染色表明,1%SDS和9小时处理能够有效地从脊髓样品中去除细胞。细胞活力测定显示脱细胞基质对PC12细胞没有细胞毒性。含有DSCT、藻酸盐和明胶的水凝胶用作生物墨水的基底,具有剪切变薄行为和低G’/G’比,在3D生物打印后具有良好的可打印性而不影响细胞活力。该生物墨水支持PC12细胞的长期存活,93%的活细胞在打印后4周存活,并刺激层粘连蛋白-1和神经丝-M的产生。因此,这种生物墨水代表了一种易于获得的用于中枢神经系统组织工程的生物材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Production of a Bioink Containing Decellularized Spinal Cord Tissue for 3D Bioprinting.

For the past few years, three-dimensional (3D) bioprinting has emerged as a promising approach in the field of regenerative medicine. This technique allows for the production of 3D scaffolds to support cell transplantation due to its ability to mimic the extracellular environment. One alternative to enhancing cell adhesion, survival, and proliferation is the use of decellularized extracellular matrix as a bioink component. The aim of this study was to produce a bioink using lyophilized rat decellularized spinal cord tissue (DSCT) for 3D bioprinting of nervous tissue. DNA quantification, hematoxylin and eosin and DAPI staining indicated that 1% sodium dodecyl sulfate and 9 h processing were effective in removing the cells from the spinal cord samples. The cell viability assay showed that the decellularized matrix is not cytotoxic for PC12 cells. The hydrogel containing DSCT, alginate, and gelatine used as the base for the bioink has a shear thinning behavior and low G″/G' ratio, allowing for good printability without compromising cell viability after 3D bioprinting. The bioink supported long-term PC12 cell survival, with 93% of live cells 4 weeks after printing, and stimulated the production of laminin-1 and neurofilament-M. This bioink, therefore, represents an easily available biomaterial for central nervous system tissue engineering.

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来源期刊
Tissue Engineering Part A
Tissue Engineering Part A Chemical Engineering-Bioengineering
CiteScore
9.20
自引率
2.40%
发文量
163
审稿时长
3 months
期刊介绍: Tissue Engineering is the preeminent, biomedical journal advancing the field with cutting-edge research and applications that repair or regenerate portions or whole tissues. This multidisciplinary journal brings together the principles of engineering and life sciences in the creation of artificial tissues and regenerative medicine. Tissue Engineering is divided into three parts, providing a central forum for groundbreaking scientific research and developments of clinical applications from leading experts in the field that will enable the functional replacement of tissues.
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