引起医院感染的blaOXA-48和blaNDM-1产生的肺炎克雷伯菌的分子流行病学分型。

IF 1.5 4区医学 Q4 MICROBIOLOGY

New Microbiologica Pub Date : 2023-09-01

Pegah Shakib, Rashid Ramazanzadeh

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引用次数: 0

摘要

在本研究中，通过rep-PCR和PFGE方法对伊朗库尔德斯坦省肺炎克雷伯菌临床分离株的抗生素敏感性模式和bla基因进行了表征。从临床样品中分离到70株肺炎克雷伯菌，检测其耐药性、碳青霉烯酶和MBL产生菌。采用聚合酶链式反应（PCR）方法对bla基因进行鉴定。通过PFGE和Rep-PCR方法对分离物进行分型。头孢噻肟（67.1%）和亚胺培南（8.6%）的耐药率最高，最低。blaNDM-1和blaOXA-48基因的检出率分别为1（1.4%）和14（20%）。通过rep-PCR将其分为27个聚类和39个PFGE类型。本研究中碳青霉烯酶和MBL基因的低频率在流行病学上具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

本刊更多论文

Molecular epidemiology typing of blaOXA-48 and blaNDM-1 producing Klebsiella pneumoniae causing nosocomial infection.

In this study the antibiotic susceptibility pattern and bla genes were characterized in Klebsiella pneumoniae clinical isolates that fingerprinted by rep-PCR and PFGE methods at Kurdistan Province, Iran. A total of 70 K. pneumoniae were isolated from clinical samples to detect the antimicrobial susceptibility, carbapenemase and MBL-producing isolates. The PCR assay was used to identify the bla genes. Isolates were typed by PFGE and Rep-PCR methods. The highest and lowest rates of resistance were observed in cefotaxime (67.1%) and imipenem (8.6%), respectively. The rate of blaNDM-1 and blaOXA-48 genes were 1 (1.4%) and 14 (20%) isolates, respectively. All were classified in 27 clusters by rep-PCR and 39 PFGE types. The low frequency of carbapenemase and MBL genes in this study are epidemiologically important.

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来源期刊

New Microbiologica 生物-微生物学

CiteScore

2.20

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The publication, diffusion and furtherance of research and study on all aspects of basic and clinical Microbiology and related fields are the chief aims of the journal.