RING-H2基因LdXERICO在低温调控下对独花百合休眠解除起着负调控作用。

IF 7.6 Q1 GENETICS & HEREDITY
园艺研究(英文) Pub Date : 2023-02-20 eCollection Date: 2023-04-01 DOI:10.1093/hr/uhad030
Xinyue Fan, Xiaoman Zou, Linlan Fu, Yue Yang, Min Li, Chunxia Wang, Hongmei Sun
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引用次数: 0

摘要

休眠调控是百合产业可持续发展的基础。因此,对百合休眠的基础研究对百合栽培育种的创新具有重要意义。先前的研究表明,休眠的解除在很大程度上取决于脱落酸(ABA)的降解。然而,关键基因和潜在的调控网络仍不清楚。我们采用外源ABA和ABA抑制剂来阐明ABA对百合休眠的影响。基于加权基因共表达网络分析(WGCNA)的结果,在与内源ABA高度相关的模块中鉴定了枢纽基因LdXERICO,并鉴定了大量共表达基因。LdXERICO由外源ABA诱导,并在具有强烈生理活性的组织中以较高水平表达。沉默LdXERICO提高了小鳞茎的低温敏感性,加速了小鳞茎发芽。LdXERICO挽救了拟南芥种子萌发过程中xerico突变体对ABA的不敏感性,表明它促进了种子休眠,并支持对百合小鳞茎的过表达研究。在表达LdXERICO的转基因拟南芥中,ABA水平显著升高,表明LdXERICO通过促进ABA合成发挥作用。我们通过在拟南芥中过表达LdICE1产生了三个转基因系,并表明,与LdXERICO相比,LdCE1正调节休眠释放。最后,qRT-PCR证实LdXERICO对LdICE1的休眠释放具有上位性。我们认为LdXERICO是通过ABA相关途径调节休眠的重要基因,具有涉及温度信号的复杂调控网络。本研究为进一步探索球茎休眠解除机制提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The RING-H2 gene <i>LdXERICO</i> plays a negative role in dormancy release regulated by low temperature in <i>Lilium davidii</i> var. <i>unicolor</i>.

The RING-H2 gene <i>LdXERICO</i> plays a negative role in dormancy release regulated by low temperature in <i>Lilium davidii</i> var. <i>unicolor</i>.

The RING-H2 gene <i>LdXERICO</i> plays a negative role in dormancy release regulated by low temperature in <i>Lilium davidii</i> var. <i>unicolor</i>.

The RING-H2 gene LdXERICO plays a negative role in dormancy release regulated by low temperature in Lilium davidii var. unicolor.

Dormancy regulation is the basis of the sustainable development of the lily industry. Therefore, basic research on lily dormancy is crucial for innovation in lily cultivation and breeding. Previous studies revealed that dormancy release largely depends on abscisic acid (ABA) degradation. However, the key genes and potential regulatory network remain unclear. We used exogenous ABA and ABA inhibitors to elucidate the effect of ABA on lily dormancy. Based on the results of weighted gene coexpression network analysis (WGCNA), the hub gene LdXERICO was identified in modules highly related to endogenous ABA, and a large number of coexpressed genes were identified. LdXERICO was induced by exogenous ABA and expressed at higher levels in tissues with vigorous physiological activity. Silencing LdXERICO increased the low-temperature sensitivity of bulblets and accelerated bulblet sprouting. LdXERICO rescued the ABA insensitivity of xerico mutants during seed germination in Arabidopsis, suggesting that it promotes seed dormancy and supporting overexpression studies on lily bulblets. The significant increase in ABA levels in transgenic Arabidopsis expressing LdXERICO indicated that LdXERICO played a role by promoting ABA synthesis. We generated three transgenic lines by overexpressing LdICE1 in Arabidopsis thaliana and showed that, in contrast to LdXERICO, LdICE1 positively regulated dormancy release. Finally, qRT-PCR confirmed that LdXERICO was epistatic to LdICE1 for dormancy release. We propose that LdXERICO, an essential gene in dormancy regulation through the ABA-related pathway, has a complex regulatory network involving temperature signals. This study provides a theoretical basis for further exploring the mechanism of bulb dormancy release.

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CiteScore
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