用于噬菌体设计和治疗应用的基因组编辑。

3区 生物学 Q2 Biochemistry, Genetics and Molecular Biology
Karthik Loganathan, Baskaran Viswanathan
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引用次数: 0

摘要

抗生素的过度使用导致抗生素滥用,进而最终提高了各种病原体的耐药性机制。由于缺乏噬菌体作为潜在抗菌和治疗剂的疗效的实验数据,以及针对特定病原体的更具体和繁琐的分离过程,因此将其视为替代疗法的技术并不可行。但是,基因组编辑技术的最新发展使程序化核酸酶能够有效地即兴发挥我们的方法,对原核生物和真核生物细胞的基因组进行准确的改变。它已经通过促进创建更精确的模型来识别根本原因,增强了我们将基因工程即兴用于疾病识别的能力。本章讨论了使用最新的基因组编辑工具进行噬菌体治疗的即兴创作,并分享了噬菌体及其衍生物(如蛋白质和酶,如赖氨酸和解聚酶)作为潜在治疗或预防剂的使用方法数据。本章还讨论了基于重组的技术中涉及的方法。将传统方法与现代工具相结合,在不久的将来有可能开发出基于噬菌体的治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genome editing for phage design and uses for therapeutic applications.

The over usage of antibiotics leads to antibiotic abuse which in turn eventually raises resistance mechanisms among wide range of pathogens. Due to lack of experimental data of efficacy of phages as potential antimicrobial and therapeutic agent and also more specific and cumbersome isolation process against specific pathogens makes it not so feasible technology to be looked as an alternative therapy. But, recent developments in genome editing techniques enables programmed nuclease enzymes that has effectively improvised our methodology to make accurate changes in the genomes of prokaryote as well as eukaryote cells. It is already strengthening our ability to improvise genetic engineering to disease identification by facilitating the creation of more precise models to identify the root cause. The present chapter discusses on improvisation of phage therapy using recent genome editing tools and also shares data on the methods of usage of phages and their derivatives like proteins and enzymes such as lysins and depolymerases, as a potential therapeutic or prophylaxis agent. Methods involved in recombinant based techniques were also discussed in this chapter. Combination of traditional approach with modern tools has led to a potential development of phage-based therapeutics in near future.

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来源期刊
CiteScore
5.00
自引率
0.00%
发文量
110
审稿时长
4-8 weeks
期刊介绍: Progress in Molecular Biology and Translational Science (PMBTS) provides in-depth reviews on topics of exceptional scientific importance. If today you read an Article or Letter in Nature or a Research Article or Report in Science reporting findings of exceptional importance, you likely will find comprehensive coverage of that research area in a future PMBTS volume.
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