Eric C. Leszczynski , Nicole E. Schwartz , Ashley C. McPeek , Katharine D. Currie , David P. Ferguson , Theodore Garland Jr.
{"title":"在雌性小鼠中选择性地繁殖高自主性体力活动并不能赋予类似心脏偏心重塑的固有特征,但小肌肉表型确实具有。","authors":"Eric C. Leszczynski , Nicole E. Schwartz , Ashley C. McPeek , Katharine D. Currie , David P. Ferguson , Theodore Garland Jr.","doi":"10.1016/j.smhs.2023.07.003","DOIUrl":null,"url":null,"abstract":"<div><p>Physical activity engagement results in a variety of positive health outcomes, including a reduction in cardiovascular disease risk partially due to eccentric remodeling of the heart. The purpose of this investigation was to determine if four replicate lines of High Runner mice that have been selectively bred for voluntary exercise on wheels have a cardiac phenotype that resembles the outcome of eccentric remodeling. Adult females (average age 55 days) from the 4 High Runner and 4 non-selected control lines were anaesthetized via vaporized isoflurane, then echocardiographic images were collected and analyzed for structural and functional differences. High Runner mice in general had lower ejection fractions compared to control mice lines (2-tailed <em>p</em> = 0.023 6) and tended to have thicker walls of the anterior portion of the left ventricle (<em>p</em> = 0.065). However, a subset of the High Runner individuals, termed mini-muscle mice, had greater ejection fraction (<em>p</em> = 0.000 6), fractional shortening percentage (<em>p</em> < 0.000 1), and ventricular mass at dissection (<em>p</em> < 0.002 7 with body mass as a covariate) compared to non-mini muscle mice. Mice from replicate lines bred for high voluntary exercise did not all have inherent positive cardiac functional or structural characteristics, although a genetically unique subset of mini-muscle individuals did have greater functional cardiac characteristics, which in conjunction with their previously described peripheral aerobic enhancements (e.g., increased capillarity) would partially account for their increased <span><math><mrow><mover><mi>V</mi><mo>˙</mo></mover></mrow></math></span> O<sub>2max</sub>.</p></div>","PeriodicalId":33620,"journal":{"name":"Sports Medicine and Health Science","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/84/ff/main.PMC10518799.pdf","citationCount":"0","resultStr":"{\"title\":\"Selectively breeding for high voluntary physical activity in female mice does not bestow inherent characteristics that resemble eccentric remodeling of the heart, but the mini-muscle phenotype does\",\"authors\":\"Eric C. Leszczynski , Nicole E. Schwartz , Ashley C. McPeek , Katharine D. Currie , David P. Ferguson , Theodore Garland Jr.\",\"doi\":\"10.1016/j.smhs.2023.07.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Physical activity engagement results in a variety of positive health outcomes, including a reduction in cardiovascular disease risk partially due to eccentric remodeling of the heart. The purpose of this investigation was to determine if four replicate lines of High Runner mice that have been selectively bred for voluntary exercise on wheels have a cardiac phenotype that resembles the outcome of eccentric remodeling. Adult females (average age 55 days) from the 4 High Runner and 4 non-selected control lines were anaesthetized via vaporized isoflurane, then echocardiographic images were collected and analyzed for structural and functional differences. High Runner mice in general had lower ejection fractions compared to control mice lines (2-tailed <em>p</em> = 0.023 6) and tended to have thicker walls of the anterior portion of the left ventricle (<em>p</em> = 0.065). However, a subset of the High Runner individuals, termed mini-muscle mice, had greater ejection fraction (<em>p</em> = 0.000 6), fractional shortening percentage (<em>p</em> < 0.000 1), and ventricular mass at dissection (<em>p</em> < 0.002 7 with body mass as a covariate) compared to non-mini muscle mice. Mice from replicate lines bred for high voluntary exercise did not all have inherent positive cardiac functional or structural characteristics, although a genetically unique subset of mini-muscle individuals did have greater functional cardiac characteristics, which in conjunction with their previously described peripheral aerobic enhancements (e.g., increased capillarity) would partially account for their increased <span><math><mrow><mover><mi>V</mi><mo>˙</mo></mover></mrow></math></span> O<sub>2max</sub>.</p></div>\",\"PeriodicalId\":33620,\"journal\":{\"name\":\"Sports Medicine and Health Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2023-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/84/ff/main.PMC10518799.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sports Medicine and Health Science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2666337623000410\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"SPORT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sports Medicine and Health Science","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2666337623000410","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"SPORT SCIENCES","Score":null,"Total":0}
Selectively breeding for high voluntary physical activity in female mice does not bestow inherent characteristics that resemble eccentric remodeling of the heart, but the mini-muscle phenotype does
Physical activity engagement results in a variety of positive health outcomes, including a reduction in cardiovascular disease risk partially due to eccentric remodeling of the heart. The purpose of this investigation was to determine if four replicate lines of High Runner mice that have been selectively bred for voluntary exercise on wheels have a cardiac phenotype that resembles the outcome of eccentric remodeling. Adult females (average age 55 days) from the 4 High Runner and 4 non-selected control lines were anaesthetized via vaporized isoflurane, then echocardiographic images were collected and analyzed for structural and functional differences. High Runner mice in general had lower ejection fractions compared to control mice lines (2-tailed p = 0.023 6) and tended to have thicker walls of the anterior portion of the left ventricle (p = 0.065). However, a subset of the High Runner individuals, termed mini-muscle mice, had greater ejection fraction (p = 0.000 6), fractional shortening percentage (p < 0.000 1), and ventricular mass at dissection (p < 0.002 7 with body mass as a covariate) compared to non-mini muscle mice. Mice from replicate lines bred for high voluntary exercise did not all have inherent positive cardiac functional or structural characteristics, although a genetically unique subset of mini-muscle individuals did have greater functional cardiac characteristics, which in conjunction with their previously described peripheral aerobic enhancements (e.g., increased capillarity) would partially account for their increased O2max.