健康人体志愿者急性和短期营养干预后金浆果(Physalis peruviana)的主要尿液生物标志物。

Fabrice Vaillant, Sandra Llano, Alberto Ángel Martín, Natalia Moreno-Castellanos
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引用次数: 0

摘要

由于食用金浆果而进入血液并在尿液中排出的代谢物目前尚未确定。然而,这些代谢产物可能是在各种体外、动物和人类模型中观察到的健康益处的基础。对18名健康人类志愿者进行营养干预,并在基线时以及急性和短期食用水果19天后收集尿液。在UPLC-ESI/QToF-MS分析后,对尿液样本进行非靶向代谢组学分析,从经验证的PLS-DA模型(CV-ANOVA=3.7e-35;R^2Y=0.86,Q^2Y=0.62且无过拟合)产生的50个最具判别性的离子(VIP>2)中,鉴定出22个具有相对高置信度的化合物。经DHS/GC-MS2尿液挥发物分析证实,最具判别力的代谢物是倍半萜(C15H22):3种立体异构体,β-缬氨酸、β-维替芬和β-维地吡喃,以及2种异构体,埃雷莫非-1(10)、8,11-三烯和α-枯烯。另一个主要的尿液生物标志物是4β-羟基己烯醇内酯E及其II期衍生物,在食用水果后24小时内,在所有个体的尿液中都能观察到这些生物标志物;因此,这种生物标志物在人体中的生物利用度首次得到证实。此外,食用金浆果后,尿液中某些酰基肉碱和次黄嘌呤的排泄量增加,这可能与解毒作用有关,可能是因为脂肪而不是碳水化合物被用来满足身体的能量需求。金浆果消费的主要生物标志物是这种水果特有的,这证实了它在功能性食品市场上的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Main urinary biomarkers of golden berries (Physalis peruviana) following acute and short-term nutritional intervention in healthy human volunteers.

The metabolites entering the bloodstream and being excreted in urine as a result of consuming golden berries are currently unidentified. However, these metabolites potentially underlie the health benefits observed in various in vitro, animal, and human models. A nutritional intervention with 18 healthy human volunteers was performed, and urine was collected at baseline and after acute and short-term fruit consumption for 19 days. After UPLC-ESI/QToF-MS analysis, untargeted metabolomics was performed on the urine samples, and from the 50 most discriminant ions (VIP > 2) generated by a validated PLS-DA model (CV-ANOVA = 3.7e-35; R^2Y = 0.86, Q^2Y = 0.62 and no overfitting), 22 compounds were identified with relatively high confidence. The most discriminant metabolites confirmed by DHS/GC-MS2 analysis of volatiles in urine were sesquiterpenes (C15H22): 3 stereoisomers, β-vatirenene, β-vetivenene, and β-vetispirene, and 2 isomers, eremophila-1(10),8,11-triene and α-curcumene. Another major urinary biomarker was 4β-hydroxywithanolide E and its phase II derivatives, which were observed in urine for all individual up to 24 h after the fruit was consumed; thus, the bioavailability of this biomarker in humans was demonstrated for the first time. Additionally, the excretion of certain acylcarnitines and hypoxanthine in urine increased after golden berry consumption, which may be associated with a detoxifying effect and may occur because fats were utilized rather than carbohydrates to meet the body's energy needs. The main biomarkers of golden berry consumption are specific to this fruit, confirming its potential for the functional food market.

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