松弛素3在日本鹌鹑卵巢卵泡中的表达。

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Journal of Poultry Science Pub Date : 2023-10-04 eCollection Date: 2023-01-01 DOI:10.2141/jpsa.2023025
Khoi X Hoang, Mei Matsuzaki, Tetsuya Kohsaka, Tomohiro Sasanami
{"title":"松弛素3在日本鹌鹑卵巢卵泡中的表达。","authors":"Khoi X Hoang, Mei Matsuzaki, Tetsuya Kohsaka, Tomohiro Sasanami","doi":"10.2141/jpsa.2023025","DOIUrl":null,"url":null,"abstract":"<p><p>The relaxin (<i>RLN</i>) gene is expressed in the reproductive tracts, such as the ovary and uterus, of mammalian species. Although RLN expression is detected in the chicken ovary, detailed clarification of the physiological role of RLN has not yet been reported. To address this issue, in the present study we aimed to examine the spatiotemporal expression and hormonal control of RLN in Japanese quail. By performing semi-quantitative and quantitative reverse transcription-polymerase chain reaction analysis, we found that <i>RLN</i> mRNA was mainly expressed in the granulosa and theca layers of the ovary. The expression level in the granulosa layer increased with the stage of follicular development. Results from granulosa layer culture experiments revealed that <i>RLN</i> mRNA expression increased with the addition of estradiol-17β, whereas the addition of progesterone suppressed <i>RLN</i> transcription. More detailed analysis indicated that <i>RLN</i> expression was highest in the stigma region of the follicle but significantly decreased as the time of the expected luteinizing hormone (LH) surge approached. Together, our findings demonstrated that the granulosa cells in the mature preovulatory follicles constitute the main source of RLN in the Japanese quail. Because <i>RLN</i> expression was highest in the stigma region and the expression dramatically decreased following the LH surge, the results further suggest that RLN may be related to tissue remodeling for the ovulation process in birds.</p>","PeriodicalId":16883,"journal":{"name":"Journal of Poultry Science","volume":null,"pages":null},"PeriodicalIF":1.8000,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/be/77/jpsa-60-2023025.PMC10545447.pdf","citationCount":"0","resultStr":"{\"title\":\"Expression of Relaxin 3 in the Ovarian Follicle of Japanese Quail.\",\"authors\":\"Khoi X Hoang, Mei Matsuzaki, Tetsuya Kohsaka, Tomohiro Sasanami\",\"doi\":\"10.2141/jpsa.2023025\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The relaxin (<i>RLN</i>) gene is expressed in the reproductive tracts, such as the ovary and uterus, of mammalian species. Although RLN expression is detected in the chicken ovary, detailed clarification of the physiological role of RLN has not yet been reported. To address this issue, in the present study we aimed to examine the spatiotemporal expression and hormonal control of RLN in Japanese quail. By performing semi-quantitative and quantitative reverse transcription-polymerase chain reaction analysis, we found that <i>RLN</i> mRNA was mainly expressed in the granulosa and theca layers of the ovary. The expression level in the granulosa layer increased with the stage of follicular development. Results from granulosa layer culture experiments revealed that <i>RLN</i> mRNA expression increased with the addition of estradiol-17β, whereas the addition of progesterone suppressed <i>RLN</i> transcription. More detailed analysis indicated that <i>RLN</i> expression was highest in the stigma region of the follicle but significantly decreased as the time of the expected luteinizing hormone (LH) surge approached. Together, our findings demonstrated that the granulosa cells in the mature preovulatory follicles constitute the main source of RLN in the Japanese quail. Because <i>RLN</i> expression was highest in the stigma region and the expression dramatically decreased following the LH surge, the results further suggest that RLN may be related to tissue remodeling for the ovulation process in birds.</p>\",\"PeriodicalId\":16883,\"journal\":{\"name\":\"Journal of Poultry Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.8000,\"publicationDate\":\"2023-10-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/be/77/jpsa-60-2023025.PMC10545447.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Poultry Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.2141/jpsa.2023025\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Poultry Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.2141/jpsa.2023025","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

摘要

松弛素(RLN)基因在哺乳动物的生殖道中表达,如卵巢和子宫。尽管在鸡卵巢中检测到RLN的表达,但尚未报道RLN的生理作用的详细阐明。为了解决这个问题,在本研究中,我们旨在检测日本鹌鹑RLN的时空表达和激素控制。通过半定量和定量逆转录聚合酶链反应分析,我们发现RLN mRNA主要在卵巢的颗粒层和卵泡膜层表达。颗粒层的表达水平随着卵泡发育阶段的增加而增加。颗粒层培养实验结果显示,RLN mRNA表达随着雌二醇-17β的添加而增加,而孕酮的添加则抑制RLN的转录。更详细的分析表明,RLN在卵泡的柱头区表达最高,但随着预期黄体生成激素(LH)激增的时间的临近,RLN的表达显著降低。总之,我们的研究结果表明,成熟排卵前卵泡中的颗粒细胞是日本鹌鹑RLN的主要来源。由于RLN在柱头区的表达最高,并且在LH激增后表达显著下降,因此研究结果进一步表明,RLN可能与鸟类排卵过程的组织重塑有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Expression of Relaxin 3 in the Ovarian Follicle of Japanese Quail.

Expression of Relaxin 3 in the Ovarian Follicle of Japanese Quail.

Expression of Relaxin 3 in the Ovarian Follicle of Japanese Quail.

Expression of Relaxin 3 in the Ovarian Follicle of Japanese Quail.

The relaxin (RLN) gene is expressed in the reproductive tracts, such as the ovary and uterus, of mammalian species. Although RLN expression is detected in the chicken ovary, detailed clarification of the physiological role of RLN has not yet been reported. To address this issue, in the present study we aimed to examine the spatiotemporal expression and hormonal control of RLN in Japanese quail. By performing semi-quantitative and quantitative reverse transcription-polymerase chain reaction analysis, we found that RLN mRNA was mainly expressed in the granulosa and theca layers of the ovary. The expression level in the granulosa layer increased with the stage of follicular development. Results from granulosa layer culture experiments revealed that RLN mRNA expression increased with the addition of estradiol-17β, whereas the addition of progesterone suppressed RLN transcription. More detailed analysis indicated that RLN expression was highest in the stigma region of the follicle but significantly decreased as the time of the expected luteinizing hormone (LH) surge approached. Together, our findings demonstrated that the granulosa cells in the mature preovulatory follicles constitute the main source of RLN in the Japanese quail. Because RLN expression was highest in the stigma region and the expression dramatically decreased following the LH surge, the results further suggest that RLN may be related to tissue remodeling for the ovulation process in birds.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Poultry Science
Journal of Poultry Science AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
2.80
自引率
13.30%
发文量
26
审稿时长
12 months
期刊介绍: The Journal of Poultry Science will publish original reports and reviews which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: breeding and genetics, nutrition and feeds, physiology, reproduction, immunology, behavior, environmental science, management and housing welfare, processing and products, and health in poultry. Submission of original articles to the Journal is open to all poultry researchers. The review articles are invited papers written by international outstanding researchers. Articles will be published in English, American style.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信