Manman Cao , Donghong Yang , Fei Wang , Beihai Zhou , Huilun Chen , Rongfang Yuan , Ke Sun
{"title":"胞外聚合物改变了二硫化钼纳米材料的物理化学性质,以减轻其对小球藻的毒性。","authors":"Manman Cao , Donghong Yang , Fei Wang , Beihai Zhou , Huilun Chen , Rongfang Yuan , Ke Sun","doi":"10.1016/j.impact.2023.100485","DOIUrl":null,"url":null,"abstract":"<div><p>Although the toxic effects of two-dimensional nanomaterials (2D-NMs) have been widely reported, the influence of extracellular polymeric substances (EPS) on the environmental fate and risk of 2D-NMs in aquatic environments is largely unknown, and the processes and mechanisms involved remain to be revealed. Herein, we investigated the impact of EPS secreted by microalgae (<em>Chlorella vulgaris</em> (<em>C. vulgaris</em>)) on the environmental transformation and risk of molybdenum disulfide (MoS<sub>2</sub>). We found that the attachment of EPS increased the thickness of MoS<sub>2</sub> (from 2 nm to 5 nm), changed it from a monolayer sheet to a fuzzy multilayer structure, and promoted the formation of defects on MoS<sub>2</sub>. The blue-shift of the peak associated with the plasmon resonances in the 1 T phase and the generation of electron-hole pairs suggested that EPS altered the surface electronic structure of MoS<sub>2</sub>. EPS interacted mainly with the S atoms on the 1 T phase, and the attachment of EPS promoted the oxidation of MoS<sub>2</sub>. The reduction in hydrodynamic diameter (<em>D</em><sub>h</sub>) and the decrease in zeta potential indicated that EPS inhibited the agglomeration behavior of MoS<sub>2</sub> and enhanced its dispersion and stability in aqueous media. Notably, EPS reduced the generation of free radicals (superoxide anion (•O<sub>2</sub><sup>−</sup>), singlet oxygen (<sup>1</sup>O<sub>2</sub>), and hydroxyl radicals (•OH<sup>−</sup>)). Furthermore, EPS mitigated the toxicity of MoS<sub>2</sub> to <em>C. vulgaris</em>, such as attenuated reduction in biomass and chlorophyll content. Compared to pristine MoS<sub>2</sub>, MoS<sub>2</sub> + BG11 + EPS exhibited weaker oxidative stress, membrane damage and lipid peroxidation. The adsorption of EPS on MoS<sub>2</sub> surface reduced the attachment sites of MoS<sub>2</sub>, making MoS<sub>2</sub> less likely to be enriched on the cell surface. The findings have significant contribution for understanding the interactions between EPS and MoS<sub>2</sub> in aquatic ecosystems, providing scientific guidance for risk assessment of 2D-NMs.</p></div>","PeriodicalId":18786,"journal":{"name":"NanoImpact","volume":"32 ","pages":"Article 100485"},"PeriodicalIF":4.7000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Extracellular polymeric substances altered the physicochemical properties of molybdenum disulfide nanomaterials to mitigate its toxicity to Chlorella vulgaris\",\"authors\":\"Manman Cao , Donghong Yang , Fei Wang , Beihai Zhou , Huilun Chen , Rongfang Yuan , Ke Sun\",\"doi\":\"10.1016/j.impact.2023.100485\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Although the toxic effects of two-dimensional nanomaterials (2D-NMs) have been widely reported, the influence of extracellular polymeric substances (EPS) on the environmental fate and risk of 2D-NMs in aquatic environments is largely unknown, and the processes and mechanisms involved remain to be revealed. Herein, we investigated the impact of EPS secreted by microalgae (<em>Chlorella vulgaris</em> (<em>C. vulgaris</em>)) on the environmental transformation and risk of molybdenum disulfide (MoS<sub>2</sub>). We found that the attachment of EPS increased the thickness of MoS<sub>2</sub> (from 2 nm to 5 nm), changed it from a monolayer sheet to a fuzzy multilayer structure, and promoted the formation of defects on MoS<sub>2</sub>. The blue-shift of the peak associated with the plasmon resonances in the 1 T phase and the generation of electron-hole pairs suggested that EPS altered the surface electronic structure of MoS<sub>2</sub>. EPS interacted mainly with the S atoms on the 1 T phase, and the attachment of EPS promoted the oxidation of MoS<sub>2</sub>. The reduction in hydrodynamic diameter (<em>D</em><sub>h</sub>) and the decrease in zeta potential indicated that EPS inhibited the agglomeration behavior of MoS<sub>2</sub> and enhanced its dispersion and stability in aqueous media. Notably, EPS reduced the generation of free radicals (superoxide anion (•O<sub>2</sub><sup>−</sup>), singlet oxygen (<sup>1</sup>O<sub>2</sub>), and hydroxyl radicals (•OH<sup>−</sup>)). Furthermore, EPS mitigated the toxicity of MoS<sub>2</sub> to <em>C. vulgaris</em>, such as attenuated reduction in biomass and chlorophyll content. Compared to pristine MoS<sub>2</sub>, MoS<sub>2</sub> + BG11 + EPS exhibited weaker oxidative stress, membrane damage and lipid peroxidation. The adsorption of EPS on MoS<sub>2</sub> surface reduced the attachment sites of MoS<sub>2</sub>, making MoS<sub>2</sub> less likely to be enriched on the cell surface. The findings have significant contribution for understanding the interactions between EPS and MoS<sub>2</sub> in aquatic ecosystems, providing scientific guidance for risk assessment of 2D-NMs.</p></div>\",\"PeriodicalId\":18786,\"journal\":{\"name\":\"NanoImpact\",\"volume\":\"32 \",\"pages\":\"Article 100485\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"NanoImpact\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2452074823000368\",\"RegionNum\":3,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"NanoImpact","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2452074823000368","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Extracellular polymeric substances altered the physicochemical properties of molybdenum disulfide nanomaterials to mitigate its toxicity to Chlorella vulgaris
Although the toxic effects of two-dimensional nanomaterials (2D-NMs) have been widely reported, the influence of extracellular polymeric substances (EPS) on the environmental fate and risk of 2D-NMs in aquatic environments is largely unknown, and the processes and mechanisms involved remain to be revealed. Herein, we investigated the impact of EPS secreted by microalgae (Chlorella vulgaris (C. vulgaris)) on the environmental transformation and risk of molybdenum disulfide (MoS2). We found that the attachment of EPS increased the thickness of MoS2 (from 2 nm to 5 nm), changed it from a monolayer sheet to a fuzzy multilayer structure, and promoted the formation of defects on MoS2. The blue-shift of the peak associated with the plasmon resonances in the 1 T phase and the generation of electron-hole pairs suggested that EPS altered the surface electronic structure of MoS2. EPS interacted mainly with the S atoms on the 1 T phase, and the attachment of EPS promoted the oxidation of MoS2. The reduction in hydrodynamic diameter (Dh) and the decrease in zeta potential indicated that EPS inhibited the agglomeration behavior of MoS2 and enhanced its dispersion and stability in aqueous media. Notably, EPS reduced the generation of free radicals (superoxide anion (•O2−), singlet oxygen (1O2), and hydroxyl radicals (•OH−)). Furthermore, EPS mitigated the toxicity of MoS2 to C. vulgaris, such as attenuated reduction in biomass and chlorophyll content. Compared to pristine MoS2, MoS2 + BG11 + EPS exhibited weaker oxidative stress, membrane damage and lipid peroxidation. The adsorption of EPS on MoS2 surface reduced the attachment sites of MoS2, making MoS2 less likely to be enriched on the cell surface. The findings have significant contribution for understanding the interactions between EPS and MoS2 in aquatic ecosystems, providing scientific guidance for risk assessment of 2D-NMs.
期刊介绍:
NanoImpact is a multidisciplinary journal that focuses on nanosafety research and areas related to the impacts of manufactured nanomaterials on human and environmental systems and the behavior of nanomaterials in these systems.