补充吡哆醇或钴胺素对人胶质母细胞瘤细胞系凋亡和细胞周期进展的影响。

IF 1.7 4区 医学 Q4 NEUROSCIENCES
International Journal of Neuroscience Pub Date : 2024-11-01 Epub Date: 2023-10-03 DOI:10.1080/00207454.2023.2263815
Carolina A Martínez-Mendiola, José A Estrada, Luis Á Zapi-Colín, Gerson G Contreras-Chávez, Irazú Contreras
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引用次数: 0

摘要

胶质母细胞瘤是最具侵袭性的脑肿瘤,目前的治疗方法未能显著提高患者的生存率。维生素对与肿瘤发展和进展相关的细胞过程具有重要影响。目的:本研究探讨补充吡哆醇或钴胺素对人胶质母细胞瘤细胞系U-87生存能力和细胞周期进展的影响 MG。方法:用增加浓度的吡哆醇或钴胺素处理细胞培养物24-72 h.补充后,通过分光光度法和流式细胞术评估细胞活力和细胞周期进展。通过蛋白质印迹分析补充的细胞中Bcl-2和活性胱天蛋白酶3的表达。结果:结果表明,补充吡哆醇以剂量和时间依赖的方式降低细胞活力。补充吡啶毒素的细胞中活力的丧失可能与细胞周期进展较少、活性胱天蛋白酶3表达较高和细胞凋亡有关。此外,Bcl-2的表达似乎没有被维生素补充所改变,但活性胱天蛋白酶3的表达在补充了吡哆醇的细胞中显著增加,而在补充了钴胺素的细胞中则没有,此外,在细胞活力测定中,当联合使用钴胺素时,可抑制吡哆醇对细胞的细胞毒性。结论:补充吡哆醇可促进人胶质母细胞瘤衍生细胞的凋亡,并可能有助于增强体内细胞毒性治疗的效果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect of pyridoxine or cobalamin supplementation on apoptosis and cell cycle progression in a human glioblastoma cell line.

Glioblastoma is the most aggressive type of brain tumor, with current therapies failing to significantly improve patient survival. Vitamins have important effects on cellular processes that are relevant for tumor development and progression.

Aim: The present study explored the effect of pyridoxine or cobalamin supplementation on the viability and cell cycle progression of human glioblastoma cell line U-87 MG.

Method: Cell cultures were treated with increasing concentrations of pyridoxine or cobalamin for 24-72 h. After supplementation, cell viability and cell cycle progression were assessed by spectrophotometry and flow cytometry. Analysis of Bcl-2 and active caspase 3 expression in supplemented cells was performed by western blot.

Result: The results show that pyridoxine supplementation decreases cell viability in a dose and time dependent manner. Loss of viability in pyridoxin-supplemented cells is probably related to less cell cycle progression, higher active caspase 3 expression and apoptosis. In addition, Bcl-2 expression did not appear to be altered by vitamin supplementation, but active caspase 3 expression was significantly increased in pyridoxine-, but not cobalamin-supplemented cells, furthermore, cobalamin inhibited the pyridoxine cytotoxicity in the cell viability assay when combined.

Conclusion: The results suggest that pyridoxine supplementation promotes apoptosis in human glioblastoma-derived cells and may be useful to enhance the effect of cytotoxic therapies in vivo.

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来源期刊
CiteScore
5.10
自引率
0.00%
发文量
132
审稿时长
2 months
期刊介绍: The International Journal of Neuroscience publishes original research articles, reviews, brief scientific reports, case studies, letters to the editor and book reviews concerned with problems of the nervous system and related clinical studies, epidemiology, neuropathology, medical and surgical treatment options and outcomes, neuropsychology and other topics related to the research and care of persons with neurologic disorders.  The focus of the journal is clinical and transitional research. Topics covered include but are not limited to: ALS, ataxia, autism, brain tumors, child neurology, demyelinating diseases, epilepsy, genetics, headache, lysosomal storage disease, mitochondrial dysfunction, movement disorders, multiple sclerosis, myopathy, neurodegenerative diseases, neuromuscular disorders, neuropharmacology, neuropsychiatry, neuropsychology, pain, sleep disorders, stroke, and other areas related to the neurosciences.
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