橙皮素对H2O2诱导的神经母细胞瘤SH-SY5Y细胞损伤的神经保护作用。

IF 2 4区 医学 Q3 NUTRITION & DIETETICS
Nutrition Research and Practice Pub Date : 2023-10-01 Epub Date: 2023-07-19 DOI:10.4162/nrp.2023.17.5.899
Ha-Rin Moon, Jung-Mi Yun
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引用次数: 0

摘要

背景/目的:氧化应激是一种导致神经细胞损伤和大量死亡的基本神经退行性疾病。神经退行性疾病是一种慢性中枢神经系统疾病,由神经元退化和丧失引起。最近的研究广泛关注使用膳食化合物治疗和预防神经退行性疾病。橙皮苷是一种苷元橙皮苷形式,具有抗炎、抗氧化和抗肿瘤等多种生理活性。然而,很少有研究考虑橙皮素的神经保护作用和机制;因此,我们的研究在过氧化氢(H2O2)处理的SH-SY5Y细胞中进行了研究。材料/方法:在不存在或存在橙皮素(10-40µM)的情况下,用H2O2(400µM)处理SH-SY5Y细胞24小时。三-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴化测定检测细胞活力,4’,6-二脒基-2-苯基吲哚染色使我们能够观察核形态变化,如染色质凝聚和凋亡核。活性氧(ROS)检测测定测定细胞内ROS的产生;Griess反应测定评估了一氧化氮(NO)的产生。Western印迹和定量聚合酶链反应定量了相应的mRNA和蛋白质。结果:随后的实验使用了各种无毒的橙皮素浓度,证实橙皮素显著降低了H2O2处理的SH-SY5Y细胞内ROS和NO的产生(P<0.05)。此外,橙皮素还抑制了H2O2诱导的炎症相关基因表达,包括白细胞介素-6、肿瘤坏死因子-α和核因子κB(NF-κB)p65的激活。此外,橙皮素抑制NF-κB易位到H2O2处理的SH-SY5Y细胞核中,并抑制丝裂原活化蛋白激酶蛋白的表达,这是一种重要的凋亡细胞死亡调节剂。H2O2处理的细胞中的各种凋亡特征,包括收缩和核凝结,都受到剂量依赖性的抑制。此外,橙皮素处理下调了Bax/Bcl-2的表达比率,并激活了哺乳动物雷帕霉素自噬途径的AMP活化蛋白激酶靶点。结论:橙皮素可激活细胞自噬,抑制细胞凋亡和炎症反应。赫斯佩列汀是一种潜在的强效膳食制剂,可减少神经退行性疾病的发作、进展和预防。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Neuroprotective effects of hesperetin on H<sub>2</sub>O<sub>2</sub>-induced damage in neuroblastoma SH-SY5Y cells.

Neuroprotective effects of hesperetin on H<sub>2</sub>O<sub>2</sub>-induced damage in neuroblastoma SH-SY5Y cells.

Neuroprotective effects of hesperetin on H<sub>2</sub>O<sub>2</sub>-induced damage in neuroblastoma SH-SY5Y cells.

Neuroprotective effects of hesperetin on H2O2-induced damage in neuroblastoma SH-SY5Y cells.

Background/objectives: Oxidative stress is a fundamental neurodegenerative disease trigger that damages and decimates nerve cells. Neurodegenerative diseases are chronic central nervous system disorders that progress and result from neuronal degradation and loss. Recent studies have extensively focused on neurodegenerative disease treatment and prevention using dietary compounds. Heseperetin is an aglycone hesperidin form with various physiological activities, such as anti-inflammation, antioxidant, and antitumor. However, few studies have considered hesperetin's neuroprotective effects and mechanisms; thus, our study investigated this in hydrogen peroxide (H2O2)-treated SH-SY5Y cells.

Materials/methods: SH-SY5Y cells were treated with H2O2 (400 µM) in hesperetin absence or presence (10-40 µM) for 24 h. Three-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assays detected cell viability, and 4',6-diamidino-2-phenylindole staining allowed us to observe nuclear morphology changes such as chromatin condensation and apoptotic nuclei. Reactive oxygen species (ROS) detection assays measured intracellular ROS production; Griess reaction assays assessed nitric oxide (NO) production. Western blotting and quantitative polymerase chain reactions quantified corresponding mRNA and proteins.

Results: Subsequent experiments utilized various non-toxic hesperetin concentrations, establishing that hesperetin notably decreased intracellular ROS and NO production in H2O2-treated SH-SY5Y cells (P < 0.05). Furthermore, hesperetin inhibited H2O2-induced inflammation-related gene expression, including interluekin-6, tumor necrosis factor-α, and nuclear factor kappa B (NF-κB) p65 activation. In addition, hesperetin inhibited NF-κB translocation into H2O2-treated SH-SY5Y cell nuclei and suppressed mitogen-activated protein kinase protein expression, an essential apoptotic cell death regulator. Various apoptosis hallmarks, including shrinkage and nuclear condensation in H2O2-treated cells, were suppressed dose-dependently. Additionally, hesperetin treatment down-regulated Bax/Bcl-2 expression ratios and activated AMP-activated protein kinase-mammalian target of rapamycin autophagy pathways.

Conclusion: These results substantiate that hesperetin activates autophagy and inhibits apoptosis and inflammation. Hesperetin is a potentially potent dietary agent that reduces neurodegenerative disease onset, progression, and prevention.

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来源期刊
Nutrition Research and Practice
Nutrition Research and Practice NUTRITION & DIETETICS-
CiteScore
3.50
自引率
4.20%
发文量
62
审稿时长
6-12 weeks
期刊介绍: Nutrition Research and Practice (NRP) is an official journal, jointly published by the Korean Nutrition Society and the Korean Society of Community Nutrition since 2007. The journal had been published quarterly at the initial stage and has been published bimonthly since 2010. NRP aims to stimulate research and practice across diverse areas of human nutrition. The Journal publishes peer-reviewed original manuscripts on nutrition biochemistry and metabolism, community nutrition, nutrition and disease management, nutritional epidemiology, nutrition education, foodservice management in the following categories: Original Research Articles, Notes, Communications, and Reviews. Reviews will be received by the invitation of the editors only. Statements made and opinions expressed in the manuscripts published in this Journal represent the views of authors and do not necessarily reflect the opinion of the Societies.
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