{"title":"伊朗南部法尔斯省Kazeroon和Jahrom区血液透析患者和健康受试者弓形虫病病例对照血清流行率调查。","authors":"Shahin Kadkhodaei, Zahra Kargar Jahromi, Ali Taghipour, Hassan Rezanezhad, Kavous Solhjoo","doi":"10.1155/2023/8251462","DOIUrl":null,"url":null,"abstract":"<p><p>Opportunistic parasites such as <i>Toxoplasma gondii</i> (<i>T. gondii</i>) are capable of causing neurological and ocular manifestations in patients undergoing hemodialysis. By designing a matched case-control study, we conducted a seromolecular survey of <i>T. gondii</i> in hemodialysis patients compared to a healthy group from Jahrom and Kazeroon cities in Fars Province, Iran. For this purpose, 75 hemodialysis patients from Kazeroon city, 75 hemodialysis patients from Jahrom city, and 75 healthy individuals were recruited for the study. The serum levels of specific immunoglobulins (IgG/IgM) in the case and control groups were evaluated using the enzyme-linked immunosorbent assay (ELISA) method. Also, buffy coat samples were used to extract genomic DNA. Then, Polymerase Chain Reaction (PCR) was accomplished using the RE and GRA6 genes of <i>T. gondii</i>. A standard questionnaire containing demographic factors was administered. Although the seroprevalence of the anti-<i>T. gondii</i> IgG antibody in hemodialysis patients from Kazeroon (18.66% (14/75)) and Jahrom (25.33% (19/75)) was higher than that in the control group (13.33% (10/75)), no statistically significant difference was observed between the case and control groups (<i>P</i> value = 0.373 from Kazeroon and <i>P</i> value = 0.354 from Jahrom). Among the studied variables, only residence (urban) was significantly associated with the anti-<i>T. gondii</i> IgG antibody in the case group from Kazeroon. Also, no IgM antibody titers and DNA of <i>T. gondii</i> were detected in the case and control groups from both cities. Although high seroprevalence of anti-<i>T. gondii</i> IgG antibody was seen in hemodialysis patients, further epidemiological studies with larger samples need to be done in Jahrom and Kazeroon cities and in other parts of Iran. It is also necessary for health officials to establish programs for the prevention and control of <i>T. gondii</i> infection in hemodialysis patients.</p>","PeriodicalId":16662,"journal":{"name":"Journal of Parasitology Research","volume":"2023 ","pages":"8251462"},"PeriodicalIF":1.4000,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560106/pdf/","citationCount":"0","resultStr":"{\"title\":\"A Case-Control Seroprevalence Survey of Toxoplasmosis in Hemodialysis Patients and Healthy Subjects in Kazeroon and Jahrom Districts in Fars Province, Southern Iran.\",\"authors\":\"Shahin Kadkhodaei, Zahra Kargar Jahromi, Ali Taghipour, Hassan Rezanezhad, Kavous Solhjoo\",\"doi\":\"10.1155/2023/8251462\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Opportunistic parasites such as <i>Toxoplasma gondii</i> (<i>T. gondii</i>) are capable of causing neurological and ocular manifestations in patients undergoing hemodialysis. By designing a matched case-control study, we conducted a seromolecular survey of <i>T. gondii</i> in hemodialysis patients compared to a healthy group from Jahrom and Kazeroon cities in Fars Province, Iran. For this purpose, 75 hemodialysis patients from Kazeroon city, 75 hemodialysis patients from Jahrom city, and 75 healthy individuals were recruited for the study. The serum levels of specific immunoglobulins (IgG/IgM) in the case and control groups were evaluated using the enzyme-linked immunosorbent assay (ELISA) method. Also, buffy coat samples were used to extract genomic DNA. Then, Polymerase Chain Reaction (PCR) was accomplished using the RE and GRA6 genes of <i>T. gondii</i>. A standard questionnaire containing demographic factors was administered. Although the seroprevalence of the anti-<i>T. gondii</i> IgG antibody in hemodialysis patients from Kazeroon (18.66% (14/75)) and Jahrom (25.33% (19/75)) was higher than that in the control group (13.33% (10/75)), no statistically significant difference was observed between the case and control groups (<i>P</i> value = 0.373 from Kazeroon and <i>P</i> value = 0.354 from Jahrom). Among the studied variables, only residence (urban) was significantly associated with the anti-<i>T. gondii</i> IgG antibody in the case group from Kazeroon. Also, no IgM antibody titers and DNA of <i>T. gondii</i> were detected in the case and control groups from both cities. Although high seroprevalence of anti-<i>T. gondii</i> IgG antibody was seen in hemodialysis patients, further epidemiological studies with larger samples need to be done in Jahrom and Kazeroon cities and in other parts of Iran. It is also necessary for health officials to establish programs for the prevention and control of <i>T. gondii</i> infection in hemodialysis patients.</p>\",\"PeriodicalId\":16662,\"journal\":{\"name\":\"Journal of Parasitology Research\",\"volume\":\"2023 \",\"pages\":\"8251462\"},\"PeriodicalIF\":1.4000,\"publicationDate\":\"2023-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10560106/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Parasitology Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2023/8251462\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Parasitology Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2023/8251462","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
A Case-Control Seroprevalence Survey of Toxoplasmosis in Hemodialysis Patients and Healthy Subjects in Kazeroon and Jahrom Districts in Fars Province, Southern Iran.
Opportunistic parasites such as Toxoplasma gondii (T. gondii) are capable of causing neurological and ocular manifestations in patients undergoing hemodialysis. By designing a matched case-control study, we conducted a seromolecular survey of T. gondii in hemodialysis patients compared to a healthy group from Jahrom and Kazeroon cities in Fars Province, Iran. For this purpose, 75 hemodialysis patients from Kazeroon city, 75 hemodialysis patients from Jahrom city, and 75 healthy individuals were recruited for the study. The serum levels of specific immunoglobulins (IgG/IgM) in the case and control groups were evaluated using the enzyme-linked immunosorbent assay (ELISA) method. Also, buffy coat samples were used to extract genomic DNA. Then, Polymerase Chain Reaction (PCR) was accomplished using the RE and GRA6 genes of T. gondii. A standard questionnaire containing demographic factors was administered. Although the seroprevalence of the anti-T. gondii IgG antibody in hemodialysis patients from Kazeroon (18.66% (14/75)) and Jahrom (25.33% (19/75)) was higher than that in the control group (13.33% (10/75)), no statistically significant difference was observed between the case and control groups (P value = 0.373 from Kazeroon and P value = 0.354 from Jahrom). Among the studied variables, only residence (urban) was significantly associated with the anti-T. gondii IgG antibody in the case group from Kazeroon. Also, no IgM antibody titers and DNA of T. gondii were detected in the case and control groups from both cities. Although high seroprevalence of anti-T. gondii IgG antibody was seen in hemodialysis patients, further epidemiological studies with larger samples need to be done in Jahrom and Kazeroon cities and in other parts of Iran. It is also necessary for health officials to establish programs for the prevention and control of T. gondii infection in hemodialysis patients.
期刊介绍：
Journal of Parasitology Research is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of basic and applied parasitology. Articles covering host-parasite relationships and parasitic diseases will be considered, as well as studies on disease vectors. Articles highlighting social and economic issues around the impact of parasites are also encouraged. As an international, Open Access publication, Journal of Parasitology Research aims to foster learning and collaboration between countries and communities.
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