Clitoria ternatea叶和花提取物的抗氧化、细胞毒性、抗菌活性和硅内分析。

IF 3.4 Q2 BIOCHEMICAL RESEARCH METHODS
Biochemistry Research International Pub Date : 2023-09-22 eCollection Date: 2023-01-01 DOI:10.1155/2023/8847876
Md Ariful Islam, Samiran Kumar Mondal, Shirmin Islam, Most Nourin Akther Shorna, Suvro Biswas, Md Salah Uddin, Shahriar Zaman, Md Abu Saleh
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引用次数: 1

摘要

传染病对全世界的人类健康构成重大威胁。为了应对这一挑战,我们对Clitoria ternatea植物的叶和花提取物进行了全面的研究。我们的研究包括对其抗菌、抗菌膜、抗氧化和细胞毒性的体外评估。此外,我们还采用了计算机筛选来确定有前景的化合物,这些化合物在开发新型抗大肠杆菌药物方面具有潜在应用前景。值得注意的是,我们的研究显示了13.00的显著抑制区 ± 1. mm(200 μg/ml)对抗大肠杆菌,显示出其强大的抗菌性能。此外,叶提取物和花提取物对金黄色葡萄球菌都表现出显著的生物膜抑制效果,抑制率分别为54%和58%。在抗氧化活性方面,C.ternatea的叶和花提取物显示出显著的DPPH自由基清除能力。具体而言,叶提取物在150的浓度下表现出62.39%的实质活性 μg/ml,而花提取物在相同浓度下达到44.08%。我们的研究还评估了对卤虾的影响,在200的剂量下,花提取物显示出93.33%的显著较高的死亡率 μg/ml。为了阐明潜在的治疗靶点,我们利用分子对接技术,重点研究了与大肠杆菌抗生素耐药性相关的acbR蛋白(5ENR)。在该分析中,从C.ternatea叶提取物中分离的化合物,即D1(CID-14478556)、D2(CID-6423376)和D3(CID-20393),显示出-8.2的结合能 kcal/mol,-6.5 kcal/mol和-6.3 kcal/mol。此外,来自花提取物的化合物E1(CID-5282761)、E2(CID-538757)和E3(CID-536762)显示出-5.4的结合能 kcal/mol,-5.3 kcal/mol和-5.1 kcal/mol。总之,从C.ternatea中提取的叶和花提取物是一种很有前途的自然资源,在对抗抗生素耐药性病原体方面具有潜在的治疗应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antioxidant, Cytotoxicity, Antimicrobial Activity, and <i>In Silico</i> Analysis of the Methanolic Leaf and Flower Extracts of <i>Clitoria ternatea</i>.

Antioxidant, Cytotoxicity, Antimicrobial Activity, and <i>In Silico</i> Analysis of the Methanolic Leaf and Flower Extracts of <i>Clitoria ternatea</i>.

Antioxidant, Cytotoxicity, Antimicrobial Activity, and <i>In Silico</i> Analysis of the Methanolic Leaf and Flower Extracts of <i>Clitoria ternatea</i>.

Antioxidant, Cytotoxicity, Antimicrobial Activity, and In Silico Analysis of the Methanolic Leaf and Flower Extracts of Clitoria ternatea.

Infectious diseases pose a significant threat to human health worldwide. To address this challenge, we conducted a comprehensive study on the leaf and flower extracts of Clitoria ternatea plants. Our research encompassed in vitro assessments of their antibacterial, antibiofilm, antioxidant, and cytotoxic properties. Additionally, we employed in silico screening to identify promising compounds with potential applications in developing novel anti-Escherichia coli medications. Notably, our investigation revealed a remarkable inhibition zone of 13.00 ± 1 mm when applying the leaf extract (200 μg/ml) against E. coli, showcasing its potent antibacterial properties. Furthermore, both the leaf and flower extracts exhibited substantial biofilm inhibition efficacy against S. aureus, with inhibition percentages of 54% and 58%, respectively. In the realm of antioxidant activity, the leaf and flower extracts of C. ternatea displayed noteworthy DPPH free radical scavenging capabilities. Specifically, the leaf extract exhibited a substantial activity of 62.39% at a concentration of 150 μg/ml, while the flower extract achieved 44.08% at the same concentration. Our study also evaluated the impact on brine shrimp, where the floral extract displayed a significantly higher mortality rate of 93.33% at a dosage of 200 μg/ml compared to the leaf extract. To elucidate potential therapeutic targets, we utilized molecular docking techniques, focusing on the acbR protein (5ENR) associated with antibiotic resistance in E. coli. In this analysis, compounds isolated from the C. ternatea leaf extract, namely D1 (CID-14478556), D2 (CID-6423376), and D3 (CID-20393), exhibited binding energies of -8.2 kcal/mol, -6.5 kcal/mol, and -6.3 kcal/mol, respectively. Additionally, compounds from the flower extract, E1 (CID-5282761), E2 (CID-538757), and E3 (CID-536762), displayed binding energies of -5.4 kcal/mol, -5.3 kcal/mol, and -5.1 kcal/mol, respectively. In conclusion, the leaf and flower extracts derived from C. ternatea represent a promising natural resource with potential therapeutic applications in combating antibiotic-resistant pathogens.

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来源期刊
Biochemistry Research International
Biochemistry Research International BIOCHEMICAL RESEARCH METHODS-
CiteScore
6.30
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27
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14 weeks
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