Felipe Dutra Rêgo, Eduardo Sérgio da Silva, Valeriana Valadares Lopes, Rafael Gonçalves Teixeira-Neto, Vinícius Silva Belo, Antônio Augusto Fonseca Júnior, Diego Andrade Pereira, Heber Paulino Pena, Márcia Dalastra Laurenti, Gabriela V Araújo, Vânia Lúcia Ribeiro da Matta, Islam Hussein Chouman, Thainá Bergantin Burrin, Carmen M Sandoval, Stella Maria Barrouin-Melo, Flaviane Alves de Pinho, Hélida Monteiro de Andrade, Ramon Vieira Nunes, Célia Maria Ferreira Gontijo, Vanete Thomaz Soccol, Donnamae Klocek, Danyil Grybchuk, Diego Henrique Macedo, Rubens Lima do Monte-Neto, Vyacheslav Yurchenko, Rodrigo Pedro Soares
{"title":"巴西东南部犬和人内脏利什曼病病例中婴儿利什曼原虫株中推定的利什曼原虫RNA病毒2（LRV2）的首次报告。","authors":"Felipe Dutra Rêgo, Eduardo Sérgio da Silva, Valeriana Valadares Lopes, Rafael Gonçalves Teixeira-Neto, Vinícius Silva Belo, Antônio Augusto Fonseca Júnior, Diego Andrade Pereira, Heber Paulino Pena, Márcia Dalastra Laurenti, Gabriela V Araújo, Vânia Lúcia Ribeiro da Matta, Islam Hussein Chouman, Thainá Bergantin Burrin, Carmen M Sandoval, Stella Maria Barrouin-Melo, Flaviane Alves de Pinho, Hélida Monteiro de Andrade, Ramon Vieira Nunes, Célia Maria Ferreira Gontijo, Vanete Thomaz Soccol, Donnamae Klocek, Danyil Grybchuk, Diego Henrique Macedo, Rubens Lima do Monte-Neto, Vyacheslav Yurchenko, Rodrigo Pedro Soares","doi":"10.1590/0074-02760230071","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Leishmania RNA virus 1 (LRV1) is commonly found in South American Leishmania parasites belonging to the subgenus Viannia, whereas Leishmania RNA virus 2 (LRV2) was previously thought to be restricted to the Old-World pathogens of the subgenus Leishmania.</p><p><strong>Objectives: </strong>In this study, we investigated the presence of LRV2 in strains of Leishmania (L.) infantum, the causative agent of visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions.</p><p><strong>Methods: </strong>A total of seventy-one isolates were screened for LRV2 using semi-nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene.</p><p><strong>Findings: </strong>We detected LRV2 in two L. infantum isolates (CUR268 and HP-EMO) from canine and human cases, respectively.</p><p><strong>Main conclusions: </strong>To the best of our knowledge, this is the first detection of LRV2 in the New World.</p>","PeriodicalId":18469,"journal":{"name":"Memorias do Instituto Oswaldo Cruz","volume":"118 ","pages":"e230071"},"PeriodicalIF":2.5000,"publicationDate":"2023-09-18","publicationTypes":"Journal 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Soares\",\"doi\":\"10.1590/0074-02760230071\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Leishmania RNA virus 1 (LRV1) is commonly found in South American Leishmania parasites belonging to the subgenus Viannia, whereas Leishmania RNA virus 2 (LRV2) was previously thought to be restricted to the Old-World pathogens of the subgenus Leishmania.</p><p><strong>Objectives: </strong>In this study, we investigated the presence of LRV2 in strains of Leishmania (L.) infantum, the causative agent of visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions.</p><p><strong>Methods: </strong>A total of seventy-one isolates were screened for LRV2 using semi-nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene.</p><p><strong>Findings: </strong>We detected LRV2 in two L. infantum isolates (CUR268 and HP-EMO) from canine and human cases, 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First report of putative Leishmania RNA virus 2 (LRV2) in Leishmania infantum strains from canine and human visceral leishmaniasis cases in the southeast of Brazil.
Background: Leishmania RNA virus 1 (LRV1) is commonly found in South American Leishmania parasites belonging to the subgenus Viannia, whereas Leishmania RNA virus 2 (LRV2) was previously thought to be restricted to the Old-World pathogens of the subgenus Leishmania.
Objectives: In this study, we investigated the presence of LRV2 in strains of Leishmania (L.) infantum, the causative agent of visceral leishmaniasis (VL), originating from different hosts, clinical forms, and geographical regions.
Methods: A total of seventy-one isolates were screened for LRV2 using semi-nested reverse transcription-polymerase chain reaction (RT-PCR) targeting the RNA-dependent RNA polymerase (RdRp) gene.
Findings: We detected LRV2 in two L. infantum isolates (CUR268 and HP-EMO) from canine and human cases, respectively.
Main conclusions: To the best of our knowledge, this is the first detection of LRV2 in the New World.
期刊介绍：
Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study.
Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome.
It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.
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