基于诱导多能干细胞(Ipscs)的肝类器官:益处和挑战。

IF 2.5 Q3 CELL BIOLOGY
Wahyunia Likhayati Septiana, Ariyani Noviantari, Radiana Dhewayani Antarianto
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引用次数: 0

摘要

肝脏是人体的主要代谢器官,具有调节人体许多生理功能的功能。大约70%的肝脏由肝细胞组成,肝细胞执行肝脏的代谢过程。当肝损伤发展为慢性疾病,如终末期肝病(ESLD)或肝硬化时,如果可用的移植器官供应不足以满足患者的需求,患者唯一的治疗选择是器官移植。寻找器官移植替代品的基本目标是使肝组织替代品更容易获得,并生产肝脏和生物人工肝组织。人类诱导多能干细胞(hiPSC)可以从胚胎体形成多个肝细胞谱系,成为成熟的肝细胞。hiPSC也显示出制造人类肝脏球体的一个很有前途的来源,并被制造成三维肝胆类器官,在某些方面,它也简要地突出了早期肝发生的重要特征。毫无疑问,细胞培养艺术已经发展到包括使用类器官技术作为在健康和疾病背景下学习人类生物学的资源。类器官本质上是一种微型器官,可以在三维基质中生长,在结构和功能上与真正的器官相似。这篇综述总结了从iPSC中分化肝细胞和以各种方式在iPSC基础上产生肝类器官的替代方案。人类iPSC向肝脏类器官的生长已经通过几种程序完成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Induced Pluripotent Stem Cells (Ipscs) Based Liver Organoid: the Benefits and Challenges.

The liver is the main metabolic organ and functions to regulate many physiological functions in the human body. Approximately 70% of liver mass consists of hepatic cells (hepatocytes), which execute the liver's metabolic processes. When liver damage progresses to a chronic condition, such as end-stage liver disease (ESLD) or cirrhosis of the liver, the patient's only option for therapy is organ transplantation if the supply of available transplanted organs is insufficient to meet the patient's needs. The fundamental objective of the search for alternatives to organ transplantation has been to make liver tissue replacement more accessible and to produce hepatic and bioartificial liver tissue. Multiple hepatic cell lineages can be formed from human-induced pluripotent stem cells (hiPSCs) from embryoid bodies to become mature hepatocytes. hiPSCs also show a promising source for manufacturing human liver spheroids and are made to produce three-dimensional hepatobiliary organoids, and in some ways, it also briefly highlights important features of early hepatogenesis. Unquestionably, the art of cell culture has evolved to include the use of organoid technology as a resource for learning human biology in the context of health and illness. Organoids are essentially miniature organs that can grow in a three-dimensional matrix to resemble genuine organs in terms of both structure and function. This review summarized alternative protocols to differentiate hepatocytes from iPSC and to produce liver organoids based on iPSC in various ways. The growth of human iPSCs into liver organoids has been accomplished using several procedures.

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来源期刊
CiteScore
5.80
自引率
0.00%
发文量
86
审稿时长
1 months
期刊介绍: Cellular Physiology and Biochemistry is a multidisciplinary scientific forum dedicated to advancing the frontiers of basic cellular research. It addresses scientists from both the physiological and biochemical disciplines as well as related fields such as genetics, molecular biology, pathophysiology, pathobiochemistry and cellular toxicology & pharmacology. Original papers and reviews on the mechanisms of intracellular transmission, cellular metabolism, cell growth, differentiation and death, ion channels and carriers, and the maintenance, regulation and disturbances of cell volume are presented. Appearing monthly under peer review, Cellular Physiology and Biochemistry takes an active role in the concerted international effort to unravel the mechanisms of cellular function.
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