褐藻楔叶藻提取物的抗癌及抗菌活性评价。

IF 2 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Journal of applied biomedicine Pub Date : 2023-09-01 Epub Date: 2023-09-22 DOI:10.32725/jab.2023.016
Nehal A H K Osman, Omniya M Abd-Elazeem, Rasha A Al-Eisa, Nahla S El-Shenawy
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引用次数: 0

摘要

目的:研究埃及红海褐藻乙醇粗提物的抗菌和抗癌性能。方法:将100g样品粉末与无水乙醇混合,在37°C的摇动培养箱中培养过夜,然后收集提取物。与商业抗生素相比,使用针对测试病原体(大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌和白色念珠菌)的良好扩散试验来测试提取物的抗菌活性。使用MTT法在MCF-7、HepG-2和HEP-2细胞系上评估抗癌活性。除了透射电子显微镜外,还使用细胞周期分析、膜联蛋白V和抗氧化酶研究了对HepG-2细胞系的抗癌作用机制。结果:该提取物的GC-MS植物成分图谱以脂肪酸为主。证明了对所有致病性大肠杆菌、金黄色葡萄球菌、字幕双歧杆菌和白色念珠菌分离株的广泛抗菌效果,尤其是与商业抗生素相比,在高浓度下。提取物能抑制被测细胞系的生长。我们观察到对HepG-2细胞的影响最为显著,提取物的浓度在抑制水平中起作用(IC50为44.6±0.6µg/ml)。在较低浓度下,提取物对Vero正常细胞系的影响可以忽略不计,在最高浓度(500µg/ml)下具有轻微毒性(90.8%的活力)。在相同浓度下,提取物对癌症细胞系的抑制率为80-92%。该提取物似乎已经证明对癌症细胞有很好的效果。它诱导程序性细胞死亡(凋亡),阻止细胞周期,并影响细胞内的氧化/抗氧化平衡,可能导致癌症细胞的抑制或消除。这些发现令人鼓舞,并可能对癌症治疗或该领域的进一步研究产生影响。提取物对细菌的作用比真菌大,对所有测试的分离株都有广泛的抗菌作用,尤其是与传统抗生素相比,在高浓度下。结论:根据研究结果,楔形H.feiformis可能是一种有价值的抗菌和抗癌化学物质来源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anticancer and antimicrobial evaluation of extract from brown algae Hormophysa cuneiformis.

Aim: We investigated the antimicrobial and anticancer properties of an ethanol crude extract of Red Sea brown alga (Hormophysa cuneiformis) from Egypt.

Methods: Extraction was achieved by mixing 100 g of sample powder with absolute ethanol, incubating at 37 °C overnight in a shaking incubator, and then collecting the extract. The extract's antimicrobial activity was tested using a well diffusion assay against the tested pathogens (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, and Candida albicans) in comparison to commercial antibiotics. Anticancer activity was assessed using MTT assay on MCF-7, HepG-2, and HEP-2 cell lines. The anticancer mechanism of action against the HepG-2 cell line was investigated using cell cycle analysis, Annexin V, and antioxidant enzymes, in addition to transmission electron microscopy.

Results: GC-MS phytoconstituent profile of the extract was dominant with fatty acids. A broad antimicrobial effect against all the pathogenic isolates of E. coli, S. aureus, B. subtitles, and C. albicans was demonstrated, especially at the high concentration in comparison to commercial antibiotics. The extract could inhibit the growth of the tested cell lines. We observed the most significant effect on HepG-2 cells, and the concentration of the extract played a role in the level of inhibition (IC50 of 44.6 ± 0.6 µg/ml). The extract had negligible effects on Vero normal cell lines at the lower concentration, with slight toxicity (90.8% viability) at the highest concentration (500 µg/ml). At this same concentration, the extract caused 80-92% inhibition of the cancer cell lines. The extract appears to have demonstrated promising effects on cancer cells. It induces programmed cell death (apoptosis), arrests the cell cycle, and affects the oxidative/antioxidant balance within the cells, potentially leading to the suppression or elimination of cancer cells. These findings are encouraging and may have implications for cancer treatment or further research in this area. More action of extract was seen against bacteria than fungi, with a wide antibacterial impact against all of the tested isolates, notably at the high concentration in comparison to conventional antibiotics.

Conclusion: According to the findings, H. cuneiformis may be a valuable source of chemicals that are both antimicrobial and anticancer.

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来源期刊
Journal of applied biomedicine
Journal of applied biomedicine PHARMACOLOGY & PHARMACY-
CiteScore
2.40
自引率
7.70%
发文量
13
审稿时长
>12 weeks
期刊介绍: Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.
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