含半胱氨酸肽对细菌微室外壳内部的功能化增强了铁和钴的负载能力。

IF 4.1 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biometals Pub Date : 2023-09-20 DOI:10.1007/s10534-023-00538-1

Gints Kalnins, Maris Bertins, Arturs Viksna, Kaspars Tars

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引用次数: 0

摘要

细菌微室（BMCs）是参与细菌细胞中几个生化过程的原核细胞器。这些细胞亚结构由二十面体外壳和包裹的酶核心组成。BMC的外壳已被提议作为一个有吸引力的平台，用于创建新型纳米材料、纳米笼和纳米反应器。在这项研究中，我们提出了一种用含半胱氨酸的短序列功能化重组GRM2型BMC外壳内腔的方法，并证明这种修饰外壳的铁和钴负载能力显著提高。这些结果还表明，钴和铁原子在BMC壳上的被动流动是可能的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Functionalization of bacterial microcompartment shell interior with cysteine containing peptides enhances the iron and cobalt loading capacity

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Functionalization of bacterial microcompartment shell interior with cysteine containing peptides enhances the iron and cobalt loading capacity

Bacterial microcompartments (BMCs) are prokaryotic organelles involved in several biochemical processes in bacterial cells. These cellular substructures consist of an icosahedral shell and an encapsulated enzymatic core. The outer shells of BMCs have been proposed as an attractive platform for the creation of novel nanomaterials, nanocages, and nanoreactors. In this study, we present a method for functionalizing recombinant GRM2-type BMC shell lumens with short cysteine-containing sequences and demonstrate that the iron and cobalt loading capacity of such modified shells is markedly increased. These results also imply that a passive flow of cobalt and iron atoms across the BMC shell could be possible.

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来源期刊

Biometals 生物-生化与分子生物学

CiteScore

5.90

自引率

8.60%

发文量

111

审稿时长

3 months

期刊介绍： BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.