军团菌的检测限值根据欧盟指令2020/2184。这会不会太宽容了?

4区 医学 Q2 Biochemistry, Genetics and Molecular Biology
G Bertolino, L Marras, V Coroneo
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引用次数: 0

摘要

引言:从环境污染的源头检测军团菌病后的军团菌问题早已为人所知。军团菌是一种存在于各种自然和人工栖息地,尤其是地表淡水中的细菌。在20°C至42°C的温暖水域中,它的浓度更高。人类最大的风险因素是医院的配水系统、医疗设备(如呼吸器、透析器、吸入器、加湿器、水、用于温和疗法的按摩设备)和牙科诊所使用的涡轮机中存在军团菌,尤其是住院患者。在欧洲议会于2020年12月16日发布的欧盟指令2020/2184中,军团菌的浓度被添加到评估人类饮用水质量时要确定的参数中。目标是提高饮用水的质量标准,减少瓶装水的消耗,从而减少塑料垃圾。世界卫生组织指出,从健康角度来看,军团菌造成的负担最大,它被列入需要仔细监测的参数,限制在1000 CFU/L以下。本报告的目的是根据我们的实验室经验评估新的欧盟指令2020/2184。材料和方法:我们的食品卫生实验室-医学科学与公共卫生部共处理了459个样本。所有统计分析均使用SPSS统计软件包(Windows版本23。SPSS,股份有限公司Chicago,IL)进行。结果和讨论:在病例发生地检查的67个结构中,考虑到100 CFU/L的参考值,35个样本显示至少有一个过旧值,只有25个结构具有高于阈值的至少一个样本。根据我们作为军团菌研究区域参考实验室的经验,从100 CFU/L增加到1000 CFU/L可能会降低警戒级别。事实上,在2017年10月至2021年10月期间,有病例的CFU/L中值为0(0-100)。尽管对军团菌进行了大量研究,但如我们的分析所示,只有少数研究将停药和由此产生的CFU/L与军团菌病的确诊病例联系起来。在与病例和集群相关的所有样本中,军团菌浓度的75°百分位数等于100 CFU/L,这使我们假设,根据最近的欧洲法规,将用于环境监测的等于1000 CFU/L的限值可能无法充分保护人群的风险,尤其是在为脆弱患者提供帮助的医疗机构中。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Detection Limits of Legionella According to the EU Directive 2020/2184. Could That Be Too Permissive?

Introduction: The problem of detecting legionella after a case of legionellosis from the source of environmental contamination has been known since a long time ago. Legionella is a bacterium present in various natural and artificial habitats and especially in surface fresh waters. It is found in greater concentration in warm waters, at temperatures between 20 °C and 42 °C. The greatest risk factor for humans is represented by the presence of Legionella in water distribution systems in hospitals, medical equipment (e.g. respirators, dialyzers, inhalers, humidifiers, water, massage equipment used in balneotherapy) and turbines used in dental practices, especially for hospitalized individuals. In the EU directive 2020/2184, issued by the European parliament on 16/12/2020, the concentration of Legionella was added to the parameters to be determined in assessing the quality of drinking water intended for human consumption. The objectives were to improve the quality standard of drinking water, reduce the consumption of bottled water and consequently reduce plastic waste. The WHO notes that Legionella causes the greatest burden from a health point of view and it is included among the parameters that require careful monitoring with a limit of less than 1000 CFU/L. The aim of this report was to evaluate the new EU directive 2020/2184 on the light of our laboratory experience.

Materials and methods: A total of 459 samples were processed at our Hygiene of food Laboratory - Department of Medical Sciences and Public Health. All statistical analyses were conducted using the SPSS statistical package (version 23 for Windows. SPSS, Inc. Chicago, Ill).

Results and discussion: Of the 67 structures examined where the cases occurred, 35 showed samples with at least one over-threshold value considering the reference value of 100 CFU/L, whereas using the new limit of 1000 CFU/L, only 25 structures resulted as having at least one sample above the threshold. In our experience as a regional reference laboratory for Legionella research, the increase from 100 CFU/L to 1,000 CFU/L could lead to a lower alert level. In fact, in the period between October 2017 and October 2021, the median value of CFU/L in presence of a case was 0 (0-100). Despite the large amount of studies on Legionella only a few relate the withdrawals and the consequent CFU/L with the confirmed cases of legionellosis, as in our analysis. The 75° percentile values of the Legionella concentration equal to 100 CFU/L in all samples associated with cases and clusters leads us to hypothesize that the limit equal to 1000 CFU/L that will be introduced for environmental monitoring as per recent European regulations may not be sufficiently protective for minimizing risk in the population, especially in healthcare facilities where fragile patients are assisted.

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来源期刊
Advances in experimental medicine and biology
Advances in experimental medicine and biology 医学-医学:研究与实验
CiteScore
5.90
自引率
0.00%
发文量
465
审稿时长
2-4 weeks
期刊介绍: Advances in Experimental Medicine and Biology provides a platform for scientific contributions in the main disciplines of the biomedicine and the life sciences. This series publishes thematic volumes on contemporary research in the areas of microbiology, immunology, neurosciences, biochemistry, biomedical engineering, genetics, physiology, and cancer research. Covering emerging topics and techniques in basic and clinical science, it brings together clinicians and researchers from various fields.
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