Yuriko Matsumiya , Mitsukuni Suenaga , Toshiaki Ishikawa , Toshifumi Kudo , Tsuyoshi Nakagawa , Kentaro Okamoto , Masanori Tokunaga , Claudia Hurtado , Yuki Yamada , Kentaro Oka , Motomichi Takahashi , Luis Francisco Lopez Kostner , Miguel Luis O'Ryan Gallardo , Hiroyuki Uetake , Yusuke Kinugasa
{"title":"脆弱拟杆菌作为癌症潜在预后因素的临床意义。","authors":"Yuriko Matsumiya , Mitsukuni Suenaga , Toshiaki Ishikawa , Toshifumi Kudo , Tsuyoshi Nakagawa , Kentaro Okamoto , Masanori Tokunaga , Claudia Hurtado , Yuki Yamada , Kentaro Oka , Motomichi Takahashi , Luis Francisco Lopez Kostner , Miguel Luis O'Ryan Gallardo , Hiroyuki Uetake , Yusuke Kinugasa","doi":"10.1016/j.anaerobe.2023.102784","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><p><span><em>Bacteroides fragilis</em></span> (<em>B. fragilis</em>) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic <em>B. fragilis</em> (ETBF), a subtype of <em>B. fragilis</em><span>, produces an enterotoxin (BFT; </span><em>B. fragilis</em><span> toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of </span><em>B. fragilis</em><span> and ETBF on the clinical outcome of colorectal cancer (CRC) remains unclear. We aim to assess whether their presence affects the outcome in patients with CRC after curative resection.</span></p></div><div><h3>Methods</h3><p>We obtained 197 pairs of matched formalin-fixed paraffin-embedded samples from cancerous and adjacent non-cancerous tissues of patients with pathological stage (pstage) II and III CRC after curative resection. The presence of <em>B. fragilis</em> and ETBF were estimated using real-time polymerase chain reaction, and recurrence-free survival (RFS) and overall survival (OS) of the patients were analyzed.</p></div><div><h3>Results</h3><p><span>16 S rRNA for </span><em>B. fragilis</em> and <em>bft</em> DNA were detected in 120 (60.9%) and 12 (6.1%) of the 197 patients, respectively. <em>B. fragilis</em>-positive patients had better RFS than <em>B. fragilis</em>-negative patients, although that was not statistically significant. In subgroup analysis, better outcomes on RFS were observed in the presence of <em>B. fragilis</em> in pstage II and left-sided CRC. The association of <em>B. fragilis</em> positivity on OS was accentuated in the depth of T4 subgroup. No significant differences were observed in RFS and OS between ETBF and non-toxigenic <em>B. fragilis</em>.</p></div><div><h3>Conclusions</h3><p>Our findings suggest that the presence of <em>B. fragilis</em> is associated with better outcomes in patients with pstage II and III CRC after curative resection.</p></div>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer\",\"authors\":\"Yuriko Matsumiya , Mitsukuni Suenaga , Toshiaki Ishikawa , Toshifumi Kudo , Tsuyoshi Nakagawa , Kentaro Okamoto , Masanori Tokunaga , Claudia Hurtado , Yuki Yamada , Kentaro Oka , Motomichi Takahashi , Luis Francisco Lopez Kostner , Miguel Luis O'Ryan Gallardo , Hiroyuki Uetake , Yusuke Kinugasa\",\"doi\":\"10.1016/j.anaerobe.2023.102784\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><p><span><em>Bacteroides fragilis</em></span> (<em>B. fragilis</em>) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic <em>B. fragilis</em> (ETBF), a subtype of <em>B. fragilis</em><span>, produces an enterotoxin (BFT; </span><em>B. fragilis</em><span> toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of </span><em>B. fragilis</em><span> and ETBF on the clinical outcome of colorectal cancer (CRC) remains unclear. We aim to assess whether their presence affects the outcome in patients with CRC after curative resection.</span></p></div><div><h3>Methods</h3><p>We obtained 197 pairs of matched formalin-fixed paraffin-embedded samples from cancerous and adjacent non-cancerous tissues of patients with pathological stage (pstage) II and III CRC after curative resection. The presence of <em>B. fragilis</em> and ETBF were estimated using real-time polymerase chain reaction, and recurrence-free survival (RFS) and overall survival (OS) of the patients were analyzed.</p></div><div><h3>Results</h3><p><span>16 S rRNA for </span><em>B. fragilis</em> and <em>bft</em> DNA were detected in 120 (60.9%) and 12 (6.1%) of the 197 patients, respectively. <em>B. fragilis</em>-positive patients had better RFS than <em>B. fragilis</em>-negative patients, although that was not statistically significant. In subgroup analysis, better outcomes on RFS were observed in the presence of <em>B. fragilis</em> in pstage II and left-sided CRC. The association of <em>B. fragilis</em> positivity on OS was accentuated in the depth of T4 subgroup. No significant differences were observed in RFS and OS between ETBF and non-toxigenic <em>B. fragilis</em>.</p></div><div><h3>Conclusions</h3><p>Our findings suggest that the presence of <em>B. fragilis</em> is associated with better outcomes in patients with pstage II and III CRC after curative resection.</p></div>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2023-10-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1075996423000938\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1075996423000938","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Clinical significance of Bacteroides fragilis as a potential prognostic factor in colorectal cancer
Introduction
Bacteroides fragilis (B. fragilis) is considered to act in an anti-inflammatory manner on the intestinal tract. On the contrary, enterotoxigenic B. fragilis (ETBF), a subtype of B. fragilis, produces an enterotoxin (BFT; B. fragilis toxin), leading to asymptomatic chronic infections and colonic tumor formation. However, the impact of B. fragilis and ETBF on the clinical outcome of colorectal cancer (CRC) remains unclear. We aim to assess whether their presence affects the outcome in patients with CRC after curative resection.
Methods
We obtained 197 pairs of matched formalin-fixed paraffin-embedded samples from cancerous and adjacent non-cancerous tissues of patients with pathological stage (pstage) II and III CRC after curative resection. The presence of B. fragilis and ETBF were estimated using real-time polymerase chain reaction, and recurrence-free survival (RFS) and overall survival (OS) of the patients were analyzed.
Results
16 S rRNA for B. fragilis and bft DNA were detected in 120 (60.9%) and 12 (6.1%) of the 197 patients, respectively. B. fragilis-positive patients had better RFS than B. fragilis-negative patients, although that was not statistically significant. In subgroup analysis, better outcomes on RFS were observed in the presence of B. fragilis in pstage II and left-sided CRC. The association of B. fragilis positivity on OS was accentuated in the depth of T4 subgroup. No significant differences were observed in RFS and OS between ETBF and non-toxigenic B. fragilis.
Conclusions
Our findings suggest that the presence of B. fragilis is associated with better outcomes in patients with pstage II and III CRC after curative resection.
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