卡那霉素噬菌体抗体的检测

IF 1 4区生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Applied Biochemistry and Microbiology Pub Date : 2023-10-02 DOI:10.1134/S0003683823050071

O. I. Guliy, S. S. Evstigneeva, S. A. Staroverov, A. S. Fomin, O. A. Karavaeva

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引用次数: 0

摘要

使用scFv片段的绵羊展示文库（Griffin.1）获得卡那霉素特异性的重组抗体，并证明了它们用于通过点免疫测定法测定卡那霉霉素的可能性。卡那霉素的最低可检测浓度为1μg/mL（可区分的标记结合而非背景）。已经表明，抗卡那霉素噬菌体抗体对卡那霉素特异性，并且不与其他抗生素（新霉素、四环素、氨苄青霉素和庆大霉素）相互作用。抗卡那霉素噬菌体抗体是单克隆抗体的一种很有前途的替代品，可用于卡那霉霉素的测定。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Phage Antibodies for Kanamycin Detection

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Phage Antibodies for Kanamycin Detection

Recombinant antibodies specific to kanamycin were obtained using a sheep display library of scFv fragments (Griffin.1) and the possibility of their use for the determination of kanamycin by dot-immunoassay was demonstrated. The minimum detectable concentration of kanamycin was 1 μg/mL (distinguishable label binding other than background). It has been shown that anti-kanamycin phage antibodies are specific for kanamycin and do not interact with other antibiotics (neomycin, tetracycline, ampicillin, and gentamicin). Anti-kanamycin phage antibodies are a promising alternative to monoclonal antibodies for use in the determination of kanamycin.

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来源期刊

Applied Biochemistry and Microbiology 生物-生物工程与应用微生物

CiteScore

1.70

自引率

12.50%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.