组蛋白变体HTB4通过Ib-bHLH转录因子介导的铁稳态的表观遗传学控制延缓叶片衰老

IF 9.4 1区生物学 Q1 Agricultural and Biological Sciences

New Phytologist Pub Date : 2023-06-02 DOI:10.1111/nph.19008

Qi Yang, Ting Wang, Jie Cao, Hou-Ling Wang, Shuya Tan, Yuan Zhang, Sanghoon Park, Hyunsoo Park, Hye Ryun Woo, Xiaojuan Li, Xinli Xia, Hongwei Guo, Zhonghai Li

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引用次数: 1

摘要

叶片衰老是一个由多种内在因素和包括营养缺乏在内的多种环境胁迫共同调控的有序过程。组蛋白变体参与调节植物生长发育。然而，它们在叶片衰老中的功能和潜在的调控机制在很大程度上仍不清楚。在这里，我们发现H2B组蛋白变体HTB4是叶片衰老的负调控因子。HTB4功能的丧失导致早期叶片衰老表型，这些表型通过功能互补得以挽救。RNA-seq分析显示，参与铁（Fe）稳态的几种Ib亚组碱性螺旋-环-螺旋（bHLH）转录因子（TF），包括bHLH038、bHLH039、bHLH100和bHLH101，在htb4突变体中被抑制，从而损害铁还原氧化酶2（FRO2）和铁调节转运蛋白（IRT1）的表达，铁吸收机制的两个重要组成部分。染色质免疫沉淀定量聚合酶链反应分析显示，HTB4可以与Ib-bHLH转录因子的启动子区结合，并通过促进活性标记H3K4me3在其转录起始位点附近的富集来增强其表达。此外，Ib-bHLH-TFs或IRT1的过表达抑制了htb4突变体的早衰表型。我们的工作建立了一种信号通路，HTB4 bHLH TFs-FRO2/IRT1-Fe稳态，它调节叶片衰老的开始和进展。

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Histone variant HTB4 delays leaf senescence by epigenetic control of Ib bHLH transcription factor-mediated iron homeostasis

Leaf senescence is an orderly process regulated by multiple internal factors and diverse environmental stresses including nutrient deficiency. Histone variants are involved in regulating plant growth and development. However, their functions and underlying regulatory mechanisms in leaf senescence remain largely unclear.
Here, we found that H2B histone variant HTB4 functions as a negative regulator of leaf senescence. Loss of function of HTB4 led to early leaf senescence phenotypes that were rescued by functional complementation. RNA-seq analysis revealed that several Ib subgroup basic helix–loop–helix (bHLH) transcription factors (TFs) involved in iron (Fe) homeostasis, including bHLH038, bHLH039, bHLH100, and bHLH101, were suppressed in the htb4 mutant, thereby compromising the expressions of FERRIC REDUCTION OXIDASE 2 (FRO2) and IRON-REGULATED TRANSPORTER (IRT1), two important components of the Fe uptake machinery.
Chromatin immunoprecipitation-quantitative polymerase chain reaction analysis revealed that HTB4 could bind to the promoter regions of Ib bHLH TFs and enhance their expression by promoting the enrichment of the active mark H3K4me3 near their transcriptional start sites. Moreover, overexpression of Ib bHLH TFs or IRT1 suppressed the premature senescence phenotype of the htb4 mutant.
Our work established a signaling pathway, HTB4-bHLH TFs-FRO2/IRT1-Fe homeostasis, which regulates the onset and progression of leaf senescence.

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来源期刊

New Phytologist PLANT SCIENCES-

CiteScore

17.60

自引率

5.30%

发文量

728

审稿时长

1 months

期刊介绍： New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.