具有突变(R22A)的视网膜胍基环化酶激活蛋白5 (GCAP5)的化学移位分配，该突变消除了二聚化并增强了环化酶的激活

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2023-05-02 DOI:10.1007/s12104-023-10129-3

Diana Cudia, Effibe O. Ahoulou, James B. Ames

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引用次数: 0

摘要

脊椎动物杆状和锥状光感受器中的视网膜膜观酰基环化酶(RetGCs)被称为观酰基环化酶激活蛋白(GCAP1-7)的神经元Ca2+传感器蛋白家族激活。来自斑马鱼光感受器的GCAP5与RetGC结合，并赋予Ca2+/Fe2+依赖性的RetGC酶活性调节，促进视觉光传导的恢复阶段。我们报道了具有R22A突变(称为GCAP5R22A)的GCAP5的NMR化学位移定位，该突变可以消除蛋白质二聚化并激活RetGC，其活性比野生型GCAP5 (BMRB No. 51,783)高3倍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Chemical shift assignments of retinal guanylyl cyclase activating protein 5 (GCAP5) with a mutation (R22A) that abolishes dimerization and enhances cyclase activation

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Chemical shift assignments of retinal guanylyl cyclase activating protein 5 (GCAP5) with a mutation (R22A) that abolishes dimerization and enhances cyclase activation

Retinal membrane guanylyl cyclases (RetGCs) in vertebrate rod and cone photoreceptors are activated by a family of neuronal Ca²⁺ sensor proteins called guanylyl cyclase activating proteins (GCAP1-7). GCAP5 from zebrafish photoreceptors binds to RetGC and confers Ca²⁺/Fe²⁺-dependent regulation of RetGC enzymatic activity that promotes the recovery phase of visual phototransduction. We report NMR chemical shift assignments of GCAP5 with a R22A mutation (called GCAP5^R22A) that abolishes protein dimerization and activates RetGC with 3-fold higher activity than that of wild type GCAP5 (BMRB No. 51,783).

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.