Marleen H.M.C. van Nuenen , Rianne A.F. de Ligt , Robert P. Doornbos , Janneke C.J. van der Woude , Ernst J. Kuipers , Koen Venema
{"title":"微生物代谢物对体外人肠上皮细胞和巨噬细胞的影响","authors":"Marleen H.M.C. van Nuenen , Rianne A.F. de Ligt , Robert P. Doornbos , Janneke C.J. van der Woude , Ernst J. Kuipers , Koen Venema","doi":"10.1016/j.femsim.2005.03.010","DOIUrl":null,"url":null,"abstract":"<div><p><span>Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses<span>. Therefore, we investigated the effects of the microbial metabolites butyrate, </span></span><em>iso</em>-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and <em>iso</em><span>-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas<span> of IBD patients suppressed the release of both cytokines when the barrier function is impaired.</span></span></p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2005-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.03.010","citationCount":"43","resultStr":"{\"title\":\"The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro\",\"authors\":\"Marleen H.M.C. van Nuenen , Rianne A.F. de Ligt , Robert P. Doornbos , Janneke C.J. van der Woude , Ernst J. Kuipers , Koen Venema\",\"doi\":\"10.1016/j.femsim.2005.03.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses<span>. Therefore, we investigated the effects of the microbial metabolites butyrate, </span></span><em>iso</em>-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and <em>iso</em><span>-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas<span> of IBD patients suppressed the release of both cytokines when the barrier function is impaired.</span></span></p></div>\",\"PeriodicalId\":12220,\"journal\":{\"name\":\"FEMS immunology and medical microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2005-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.femsim.2005.03.010\",\"citationCount\":\"43\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"FEMS immunology and medical microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0928824405001082\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS immunology and medical microbiology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0928824405001082","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The influence of microbial metabolites on human intestinal epithelial cells and macrophages in vitro
Microbial metabolites may influence the metabolic integrity of intestinal epithelial cells and induce mucosal immune responses. Therefore, we investigated the effects of the microbial metabolites butyrate, iso-valerate, and ammonium on Caco-2 cells and macrophages. Barrier functioning was determined by measuring transepithelial electrical resistance and basolateral recoveries of metabolites. The barrier function of Caco-2 cells remained intact after exposures. Basolateral recoveries ranged from 6.2% to 15.2%. Tumour necrosis factor-α and interleukin-10 were measured to determine immune reactions. The Caco-2 cells did not secrete both cytokines. Physiological concentrations of butyrate and iso-valerate stimulated the secretion of tumour necrosis factor-α and suppressed the secretion of interleukin-10 by macrophages that are not protected by an epithelial barrier. In contrast, ammonium concentrations as high as those produced by microbiotas of IBD patients suppressed the release of both cytokines when the barrier function is impaired.
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