[人核糖核酸酶抑制剂变体在大肠杆菌和家蚕昆虫细胞中的表达]。

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica Pub Date : 2003-10-01

Guang-Hua Huang, Guan-Zhen Yang, Jiang-Ye Chen, Xiang-Fu Wu

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摘要

核糖核酸酶抑制剂(RI)是一种酸性50 kD蛋白，含有高含量的亮氨酸和半胱氨酸残基。RI抑制胰腺型rnase。采用聚合酶链式反应(PCR)从人胎儿肝脏cDNA文库中克隆了一个RI变异。与已报道的RI相比，RIv氨基酸序列仅发现Arg(359)Ala和Leu(365)Pro两个变异。重组RIv在大肠杆菌和家蚕昆虫细胞中均有表达。在体外系统中，重组RIv对rna酶A的核糖核裂解活性有抑制作用。

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[Expression of a human ribonuclease inhibitor variant in Escherichia coli and silkworm insect cell (Bombyx mori)].

Ribonuclease inhibitor (RI) is an acidic 50 kD protein with a high content of leucine and cysteine residues. RI inhibits RNases of the pancreatic type. A variant of RI was cloned from human fetal liver cDNA library by polymerase chain reaction (PCR). Compared with the reported RI, only two variations Arg(359)Ala and Leu(365)Pro were found in RIv amino acid sequence. Recombinant RIv has been expressed both in Escherichia coli and silkworm insect cells (Bombyx mori). The recombinant RIv exhibited inhibition activity on ribonucleolytic activity of RNase A in vitro system.

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Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica

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