修饰DNA系住多个偶氮苯的SP6 RNA聚合酶对转录反应的有效光调控。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI:10.1093/nass/3.1.265

Mingzhe Liu, Hiroyuki Asanuma, Makoto Komiyama

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引用次数: 3

摘要

利用光响应性SP6启动子捆绑两个偶氮苯，实现了SP6 RNA聚合酶(RNAP)对转录反应的有效光调控。在SP6启动子的TATA或RNAP结合区内，只要有一个偶氮苯，光调控活性就很小。但当两个偶氮苯被引入到这两个区域时，转录得到了有效的光调控:在紫外线照射下的转录速度比在黑暗下快3.5倍。

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Effective photo-regulation of transcription reaction by SP6 RNA polymerase with modified DNA tethering multiple azobenzenes.

Effective photo-regulation of transcription reaction by SP6 RNA polymerase (RNAP) was achieved with photo-responsive SP6 promoter tethering two azobenzenes. With one azobenzene in either TATA or RNAP binding region of the SP6 promoter, photo-regulation activity was very small. But when two azobenzenes were introduced into both regions, efficient photo-regulation of transcription was attained: transcription proceeded 3.5 fold faster under UV irradiation than under dark.

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Nucleic acids research. Supplement (2001)

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