同源枯草芽孢杆菌基因组重组转移的应用。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI:10.1093/nass/3.1.295

Satoshi Tomita, Kenji Tsuge, Yo Kikuchi, Mitsuhiro Itaya

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引用次数: 0

摘要

采用枯草芽孢杆菌重组转移(BReT)系统对枯草芽孢杆菌指定基因组区域进行了分离研究。在BReT载体上克隆了精确基因组区域两侧的两个DNA序列。BReT质粒以高保真度恢复了预测的100 kb的基因组序列。结果表明，最初用于恢复枯草芽孢杆菌基因组载体中克隆的非同源片段的BReT系统可以应用于同源序列。

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Application of recombination transfer to the cognate Bacillus subtilis genome.

Isolation of the designated genome region of Bacillus subtilis was investigated using a B. subtilis recombinational transfer (BReT) system. Two DNA sequences flanking the precise genome region are cloned in the BReT vector. The BReT plasmid recovered the predicted genome sequence as large as 100 kb with high fidelity. The result indicates that the BReT system originally developed to recover the non-cognate segments cloned in the B. subtilis genome vector can be applied to the cognate sequence.

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Nucleic acids research. Supplement (2001)

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