人类tRNA加工缺陷变异(Val)的分析。

Nucleic acids research. Supplement (2001) Pub Date : 2003-01-01 DOI:10.1093/nass/3.1.283

Yoshio Kato, Masayuki Sano, Kazunari Taira

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引用次数: 2

摘要

真核生物的trna被转录、加工并从细胞核输出到细胞质，参与蛋白质的生物合成。人类tRNA基因(Val)的一种变体HtV4既不加工其5'-也不加工其3'-侧翼序列。通过RT-PCR分析和原位杂交，我们证实HtV4在HeLa细胞中不经加工而组成性表达，并且定位于细胞核中。

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Analysis of processing-defective variants of human tRNA(Val).

Eukaryotic tRNAs are transcribed, processed and exported from the nucleus to the cytoplasm to participate in protein biosynthesis. A variant of human gene for tRNA(Val), HtV4, is not processed neither its 5'- nor its 3'-flanking sequences. By RT-PCR analysis and in situ hybridization, we demonstrated that the HtV4 was constitutively expressed without processing, and localized in the nucleus in HeLa cells.

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Nucleic acids research. Supplement (2001)

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