伊比利亚半岛蝙蝠物种中病毒病原体的研究:新冠状病毒遗传变异的鉴定。

IF 3.2 Q1 VETERINARY SCIENCES
Alberto Moraga-Fernández, Marta Sánchez-Sánchez, João Queirós, Ana M Lopes, Joaquín Vicente, Xosé Pardavila, Jorge Sereno-Cadierno, Paulo C Alves, José de la Fuente, Isabel G Fernández de Mera
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引用次数: 2

摘要

长期以来,蝙蝠与多种病原体有关,包括影响人类的病毒,如亨尼帕病毒、丝状病毒、布尼亚病毒和冠状病毒。冠状病毒属和冠状病毒属可以感染大多数哺乳动物。其中,在过去二十年中引起三次大流行的冠状病毒SARS-CoV、MERS-CoV和SARS-CoV-2被认为起源于蝙蝠。本研究对来自伊比利亚半岛19个地点的22种蝙蝠的194份口腔拭子进行了分析,并采用三种不同的PCR检测方法(冠状病毒通用实时RT-PCR、多重常规PCR和SARS-CoV-2特异性实时RT-PCR)对其进行了表征。冠状病毒通用PCR筛查显示,194份口腔拭子中有102份呈阳性。然后用多重PCR元条形码扩增出15份阳性样本。本研究检测到的冠状病毒大部分属于α-冠状病毒属,在同一只蝙蝠中,通过多达5种不同的遗传变异鉴定出的多种冠状病毒共存。经系统发育分析,在一株株中检出的一份阳性样本为普通冠状病毒PCR和特异性SARS-CoV-2 PCR阳性,鉴定为乙型冠状病毒。这些结果支持冠状病毒的快速进化,可能通过共同感染和重组产生新的基因组潜在致病性变异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A study of viral pathogens in bat species in the Iberian Peninsula: identification of new coronavirus genetic variants.

A study of viral pathogens in bat species in the Iberian Peninsula: identification of new coronavirus genetic variants.

A study of viral pathogens in bat species in the Iberian Peninsula: identification of new coronavirus genetic variants.

A study of viral pathogens in bat species in the Iberian Peninsula: identification of new coronavirus genetic variants.

Bats have long been associated with multiple pathogens, including viruses affecting humans such as henipaviruses, filoviruses, bunyaviruses and coronaviruses. The alpha and beta coronaviruses genera can infect most mammalian species. Among them, betacoronavirus SARS-CoV, MERS-CoV and SARS-CoV-2, which have caused the three major pandemics in the last two decades, have been proposed to originate in bats. In this study, 194 oral swabs from 22 bats species sampled in 19 locations of the Iberian Peninsula were analysed and characterized by three different PCR tests (coronavirus generic real-time RT-PCR, multiplex conventional PCR, and SARS-CoV-2 specific real-time RT-PCR) to detect bat coronaviruses. Screening with coronavirus generic PCR showed 102 positives out of 194 oral swabs analysed. Then, metabarcoding with multiplex PCR amplified 15 positive samples. Most of the coronaviruses detected in this study belong to alphacoronavirus (α-CoV) genus, with multiple alphacoronaviruses identified by up to five different genetic variants coexisting in the same bat. One of the positive samples identified in a Miniopterus schreibersii bat positive for the generic coronavirus PCR and the specific SARS-CoV-2 PCR was classified as betacoronavirus (-CoV) through phylogenetic analysis. These results support the rapid evolution of coronaviruses to generate new genomic potentially pathogenic variants likely through co-infection and recombination.

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来源期刊
CiteScore
4.80
自引率
0.00%
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12
审稿时长
24 weeks
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