激素诱导的母羊发情期宫颈松弛:宫颈转位的可行性和使用 hCG 挽救黄体功能受损的母羊。

IF 17.7 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Accounts of Chemical Research Pub Date : 2024-10-01 Epub Date: 2022-11-07 DOI:10.1080/10888705.2022.2141576
Maria Emilia Franco Oliveira, Aline Matos Arrais, Gabriel Brun Vergani, Sergio Novita Esteves, Verônica Schinaider Do Amaral Pereira, Alexandre Rossetto Garcia, Rosemary Bastos, Marco Roberto Bourg Melo, Bruna Rios Coelho Alves, Jeferson Ferreira Fonseca
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引用次数: 0

摘要

对发情期的母羊进行宫颈松弛(CR)试验,以探索非手术胚胎移植(NSET)的可行方案。在试验 1 中,自然交配的母羊(n=13)接受了仅使用苯甲酸雌二醇(EB,1 毫克,D6 日)和催产素(OT,50 IU,D7 日)(G-EB+OT)或与人绒毛膜促性腺激素(hCG,300 IU,D7 日,G-EB+OT+hCG)的宫颈松弛方案,并与未使用激素的母羊(G-对照组)进行了比较。雌二醇浓度增加(P
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hormonal-Induced Cervical Relaxation During Diestrus in Ewes: Cervical Transposing Feasibility and Use of hCG for Rescuing Disrupted Luteal Function.

Cervical relaxation (CR) was performed in ewes during diestrus, to prospect a feasible protocol for non-surgical embryo transfer (NSET). In Trial 1, naturally mated ewes (n=13) received CR protocols with estradiol benzoate (EB, 1 mg on D6) and oxytocin (OT, 50 IU on D7) only (G-EB+OT) or associated with human chorionic gonadotropin (hCG, 300 IU on D7, G-EB+OT+hCG) and were compared to non-hormonally treated (G-control) ewes. Estradiol concentration increased (P<0.05) from D6 to D7 in G-EB+OT and G-EB+OT+hCG, then decreased (P<0.05) on D8. The G-EB+OT had reduced (P<0.05) progesterone on D8, and 0% (0/5) pregnancy, while G-control had 100% (3/3) conception rate. The treatment with hCG mitigated the progesterone drop on D8 and resulted in 50% (2/4) conception rate. In Trial 2, cervical transposing success after treatment with EB, OT, and hCG on D6 and D7 was assessed in nulliparous (n=25) and multiparous (n=30) ewes. The EB+OT+hCG protocol allowed efficient cervical transposition (~78% of cervical transposition success into ~5 min) in nulliparous and multiparous females and did not alter total and blood perfusion areas of corpora lutea (CL). The use of EB+OT+hCG was efficient to ensure effective cervical transposing in nulliparous and multiparous ewes, with no apparent deleterious effect on CL function, and may contribute to animal welfare, allowing a less invasive NSET procedure.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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