Filiz Kutluyer Kocabaş, Mehmet Kocabaş, Önder Aksu, Yeliz Çakir Sahilli
{"title":"抗坏血酸改善虹鳟(Oncorhynchus mykiss)抗砷毒性的精子质量:对氧化应激、生育能力和胚胎发育的影响。","authors":"Filiz Kutluyer Kocabaş, Mehmet Kocabaş, Önder Aksu, Yeliz Çakir Sahilli","doi":"10.1080/26896583.2022.2060036","DOIUrl":null,"url":null,"abstract":"<p><p>Arsenic (As) is a heavy metal and aquatic pollutant and adversely impacts the reproduction of male fish. As a chain-breaking antioxidant, ascorbic acid (AA) has high water solubility and low toxicity. In this context, the current study was performed to assess the protective role of AA (1 mM) on the sperm cells of the rainbow trout (<i>Oncorhynchus mykiss</i>) exposed to sublethal concentrations of As (8, 16 and 32 mg/L). Sperm quality parameters were analyzed using a sperm class analyzer system. Lipid peroxidation and antioxidant enzyme levels were used as indicators of oxidative stress. The fertilization, eyeing and hatching rates were determined as gamete markers. Reduced sperm quality parameters and fertility capacity resulted from <i>in vitro</i> exposure to As (P < 0.05). The oxidative stress in sperm cells increased after As exposure (P < 0.05). The presence of AA improved sperm movement parameters and fertility potential (P < 0.05). Overall, AA had a positive effect on oxidative stress and fertility ability against As toxicity and AA supplementation ameliorated detrimental effects of As in sperm cells.</p>","PeriodicalId":53200,"journal":{"name":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","volume":" ","pages":"119-132"},"PeriodicalIF":1.2000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Ascorbic acid ameliorated the sperm quality of rainbow trout (<i>Oncorhynchus mykiss</i>) against arsenic toxicity: Impact on oxidative stress, fertility ability and embryo development.\",\"authors\":\"Filiz Kutluyer Kocabaş, Mehmet Kocabaş, Önder Aksu, Yeliz Çakir Sahilli\",\"doi\":\"10.1080/26896583.2022.2060036\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Arsenic (As) is a heavy metal and aquatic pollutant and adversely impacts the reproduction of male fish. As a chain-breaking antioxidant, ascorbic acid (AA) has high water solubility and low toxicity. In this context, the current study was performed to assess the protective role of AA (1 mM) on the sperm cells of the rainbow trout (<i>Oncorhynchus mykiss</i>) exposed to sublethal concentrations of As (8, 16 and 32 mg/L). Sperm quality parameters were analyzed using a sperm class analyzer system. Lipid peroxidation and antioxidant enzyme levels were used as indicators of oxidative stress. The fertilization, eyeing and hatching rates were determined as gamete markers. Reduced sperm quality parameters and fertility capacity resulted from <i>in vitro</i> exposure to As (P < 0.05). The oxidative stress in sperm cells increased after As exposure (P < 0.05). The presence of AA improved sperm movement parameters and fertility potential (P < 0.05). Overall, AA had a positive effect on oxidative stress and fertility ability against As toxicity and AA supplementation ameliorated detrimental effects of As in sperm cells.</p>\",\"PeriodicalId\":53200,\"journal\":{\"name\":\"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis\",\"volume\":\" \",\"pages\":\"119-132\"},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1080/26896583.2022.2060036\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/4/25 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1080/26896583.2022.2060036","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/4/25 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Ascorbic acid ameliorated the sperm quality of rainbow trout (Oncorhynchus mykiss) against arsenic toxicity: Impact on oxidative stress, fertility ability and embryo development.
Arsenic (As) is a heavy metal and aquatic pollutant and adversely impacts the reproduction of male fish. As a chain-breaking antioxidant, ascorbic acid (AA) has high water solubility and low toxicity. In this context, the current study was performed to assess the protective role of AA (1 mM) on the sperm cells of the rainbow trout (Oncorhynchus mykiss) exposed to sublethal concentrations of As (8, 16 and 32 mg/L). Sperm quality parameters were analyzed using a sperm class analyzer system. Lipid peroxidation and antioxidant enzyme levels were used as indicators of oxidative stress. The fertilization, eyeing and hatching rates were determined as gamete markers. Reduced sperm quality parameters and fertility capacity resulted from in vitro exposure to As (P < 0.05). The oxidative stress in sperm cells increased after As exposure (P < 0.05). The presence of AA improved sperm movement parameters and fertility potential (P < 0.05). Overall, AA had a positive effect on oxidative stress and fertility ability against As toxicity and AA supplementation ameliorated detrimental effects of As in sperm cells.