HILIC-HPLC-ELSD法定量分析的相对校正因子计算——以巴戟天8种低聚果糖为例

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2022-08-12 eCollection Date: 2022-01-01 DOI:10.1155/2022/8022473
Lihong Zhou, Hui Ni, Linlin Zhang, Wenyong Wu, Tengqian Zhang, Qi Su, Jing Zhou, Huali Long, Jinjun Hou, Jiyu Gong, Wanying Wu
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引用次数: 0

摘要

目的:由于蒸发光散射检测器(ELSD)的响应呈非线性模式,其相对校正因子(RCF)的计算方法尚无共识,限制了LC-ELSD在单标记多组分定量分析(QAMS)中的应用。方法:以8种低聚果糖为例,以nystose (GF3)为单一标准,建立QAMS方法,同时采用HILIC-HPLC-ELSD法测定其他7种低聚果糖。研究了6种RCF的计算方法,以选择最合理的计算方法。以30批样品中QAMS与外标法(ESM)含量的相对误差作为评价6种方法的指标。最后,进行化学计量学分析,找出MO及其三种加工产品之间的差异成分。结果:首次报道了LC-ELSD法计算RCF只有一种计算方法是科学的。GF3与其他7种低聚果糖(GF1-GF8)的rcf分别为0.86、0.91、0.93、1.05、1.15、1.12和1.18。马桑8种低聚果糖的质量质量体系具有良好的线性关系(r2 > 0.9998),准确度在95 ~ 105%之间(RSD为95 ~ 105%)。马桑及其3种加工产品可根据8种低聚果糖的含量进行区分。结论:建立科学的RCF计算方法对药典中QAMS方法的建立具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of <i>Morinda Officinalis</i> as a Case Study.

Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of <i>Morinda Officinalis</i> as a Case Study.

Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of <i>Morinda Officinalis</i> as a Case Study.

Calculating Relative Correction Factors for Quantitative Analysis with HILIC-HPLC-ELSD Method: Eight Fructooligosaccharides of Morinda Officinalis as a Case Study.

Objective: Because the response of evaporating light scattering detector (ELSD) being in a nonlinear mode, there is no consensus on the method of calculating its relative correction factors (RCF), which limits the application of the quantitative analysis for multi-components by a single marker (QAMS) with LC-ELSD.

Methods: Using eight fructooligosaccharides of Morinda officinalis as a case study, the nystose (GF3) as a single standard was adopted to develop a QAMS method to simultaneously determine the other seven fructooligosaccharides with HILIC-HPLC-ELSD method. Six calculation methods of RCF were investigated to select the most reasonable method. The relative error of content between the QAMS and the external standard method (ESM) obtained from 30 batches of samples was used as an indicator to evaluate the six methods. Finally, a chemometrics analysis was performed to find the differential components among MO and its three processing products.

Results: It was first reported that only one calculation method was scientific for calculating RCF for the LC-ELSD method. The RCFs of GF3 to the other seven fructooligosaccharides (GF1-GF8) were obtained as 0.86, 0.91, 0.93, 1.05, 1.15, 1.12, and 1.18, respectively. The QAMS of eight fructooligosaccharides of Morinda officinalis was validated with good linearity (R 2 > 0.9998) and accepted the accuracy of 95-105% (RSD < 1.81%) based on nystose. Finally, Morinda officinalis and its three processed products were distinguished and could be differed based on the content of the eight fructooligosaccharides.

Conclusion: The scientific calculation method of RCF would be of great significance for developing the QAMS method in Pharmacopoeia when performing the LC-ELSD method.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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