幽门螺杆菌Thioredoxin1可能通过IL6/STAT3通路发挥高致病性作用。

IF 2 4区医学 Q3 GASTROENTEROLOGY & HEPATOLOGY

Gastroenterology Research and Practice Pub Date : 2022-08-01 eCollection Date: 2022-01-01 DOI:10.1155/2022/3175935

Yanlei Guo, Shigang Ding

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引用次数: 2

摘要

背景:最近的研究表明，在西方人群中，CagA被认为是幽门螺杆菌(HP)的高致病性。然而，在东亚地区，CagA阳性HP可达90%，但并非所有患者都会导致胃癌。本课题组发现HP thioredoxin1 (Trx1)可能是高致病性的标志。本文研究HP Trx1是否具有高致病性及其内部分子机制。材料与方法:分别构建高trx1 HP和低trx1 HP菌株与胃上皮细胞系共培养体系，检测HP菌株的影响。采用AM/PI染色，荧光显微镜观察细胞死亡情况。收集细胞上清或沉淀物检测il - 6的表达。收集细胞沉淀物，western blot检测p-STAT3的表达。此外，收集细胞上清液检测8-OHDG的表达，以研究DNA损伤程度。结果:与低trx1 HP组相比，高trx1 HP组的GES-1细胞死亡率更高(高trx1 HP(4.53±0.56)%，低trx1 HP(0.39±0.10)%，P < 0.001)。在HP感染期间，AGS和GES-1细胞中IL-6的mRNA和蛋白水平均升高，且高trx1 HP组IL-6的表达远高于低trx1 HP组。p-STAT3在hp阳性的胃黏膜中表达较高。与低trx1 HP组相比，高trx1 HP组p-STAT3的表达明显上调。8-OHDG在高trx1组的表达明显高于低trx1组(高trx1 HP(5.47±1.73)ng/ml，低trx1 HP(2.89±1.72)ng/ml, P < 0.05)。结论:HP Trx1可能是HP高致病性的标志，高Trx1的HP可通过IL6/STAT3途径介导HP感染的致病过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Helicobacter pylori Thioredoxin1 May Play a Highly Pathogenic Role via the IL6/STAT3 Pathway.

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Helicobacter pylori Thioredoxin1 May Play a Highly Pathogenic Role via the IL6/STAT3 Pathway.

Background: Recent studies have shown that CagA is considered highly pathogenic to helicobacter pylori (HP) in Western populations. However, in East Asia, CagA positive HP can be up to 90%, but not all patients will lead to gastric cancer. Our research group has found that HP thioredoxin1 (Trx1) may be a marker of high pathogenicity. Here, we investigate whether HP Trx1 exerts high pathogenicity and its internal molecular mechanism.

Materials and methods: We constructed the coculture system of high-Trx1 HP and low-Trx1 HP strains with gastric epithelial cell lines separately and detected the influence of HP strains. The cells were stained by AM/PI, and the cell's mortality was assessed by fluorescence microscope. The cell's supernatants or precipitates were collected to detect the expression of IL6. In addition, the cell's precipitates were collected, and the expression of p-STAT3 was detected by western blot. Furthermore, the cell's supernatants were collected for detecting the expression of 8-OHDG to investigate the extent of DNA damage.

Results: The high-Trx1 HP can cause higher mortality of GES-1 cells compared with the low-Trx1 HP group (high-Trx1 HP (4.53 ± 0.56) %, low-Trx1 HP (0.39 ± 0.10) %, P < 0.001). The mRNA and protein level of IL-6 in AGS and GES-1 cells were increased during HP infection, and the expression of IL-6 in the High-Trx1 HP group was much higher than the low-Trx1 HP group. Besides, the expression of p-STAT3 was higher in the HP-positive gastric mucosa. And the expression of p-STAT3 in the high-Trx1 HP group was significantly upregulated compared with the low-Trx1 HP group. Furthermore, the expression of 8-OHDG in the high-Trx1 group was much higher than the low-Trx1 group (high-Trx1 HP (5.47 ± 1.73) ng/ml, low-Trx1 HP (2.89 ± 1.72) ng/ml, P < 0.05).

Conclusion: HP Trx1 may play as a marker of high pathogenicity, and the high-Trx1 HP could mediate the pathogenic process of HP infection via the IL6/STAT3 pathway.

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来源期刊

Gastroenterology Research and Practice GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

4.40

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Gastroenterology Research and Practice is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on all areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis and therapy of gastrointestinal diseases. The aim of the journal is to provide cutting edge research related to the field of gastroenterology, as well as digestive diseases and disorders. Topics of interest include: Management of pancreatic diseases Third space endoscopy Endoscopic resection Therapeutic endoscopy Therapeutic endosonography.