{"title":"用深共熔溶剂提取茯苓多糖的结构和理化性质。","authors":"Xiaona Zhai, Weida Zhang, Haisheng Pei, Guogang Chen","doi":"10.1007/s10719-022-10073-9","DOIUrl":null,"url":null,"abstract":"<p><p>Poria cocos, a famous traditional Chinese medicine and a well-known food or food supplement, has shown therapeutic potential against cancer and the uneasiness of the mind. In addition, polysaccharides (PCPs) in this fungus were found to be various bioactive. In this work, one such PCP, PCP-1, extracted by deep eutectic solvent (DES) and separated using Sephadex G-15 columns, was characterized using GC-MS, HPGPC, FT-IR, and NMR, while also tested for physicochemical properties. Results indicated that PCP-1 contained 96.89 ± 3.21% total sugars and was a glucan with molecular weight of 3.2 kD. The main glycosidic linkage was 1,3-linked Glcp with 96.82 mol% content and a triple helix structure, and β-D-Glcp-(1 → linkage connected to the main chain through an O-6 atom was the backbone structure. In terms of the physicochemical property, PCP-1 was soluble in water, but not in organic solvent, and processed a relative high water-holding capacity (8.64 ± 0.14 g/g) and low oil-holding capacity (2.52 ± 0.21 g/g). In addition, in vitro, PCP-1 was found to have the ability of scavenging DPPH, hydroxyl free radical, superoxide anion radical and reducing ferric at different levels. This research would be useful for the further application of PCP-1.</p>","PeriodicalId":12762,"journal":{"name":"Glycoconjugate Journal","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":"{\"title\":\"Structure and physicochemical properties of polysaccharides from Poria cocos extracted by deep eutectic solvent.\",\"authors\":\"Xiaona Zhai, Weida Zhang, Haisheng Pei, Guogang Chen\",\"doi\":\"10.1007/s10719-022-10073-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Poria cocos, a famous traditional Chinese medicine and a well-known food or food supplement, has shown therapeutic potential against cancer and the uneasiness of the mind. In addition, polysaccharides (PCPs) in this fungus were found to be various bioactive. In this work, one such PCP, PCP-1, extracted by deep eutectic solvent (DES) and separated using Sephadex G-15 columns, was characterized using GC-MS, HPGPC, FT-IR, and NMR, while also tested for physicochemical properties. Results indicated that PCP-1 contained 96.89 ± 3.21% total sugars and was a glucan with molecular weight of 3.2 kD. The main glycosidic linkage was 1,3-linked Glcp with 96.82 mol% content and a triple helix structure, and β-D-Glcp-(1 → linkage connected to the main chain through an O-6 atom was the backbone structure. In terms of the physicochemical property, PCP-1 was soluble in water, but not in organic solvent, and processed a relative high water-holding capacity (8.64 ± 0.14 g/g) and low oil-holding capacity (2.52 ± 0.21 g/g). In addition, in vitro, PCP-1 was found to have the ability of scavenging DPPH, hydroxyl free radical, superoxide anion radical and reducing ferric at different levels. This research would be useful for the further application of PCP-1.</p>\",\"PeriodicalId\":12762,\"journal\":{\"name\":\"Glycoconjugate Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2022-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Glycoconjugate Journal\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10719-022-10073-9\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2022/7/16 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Glycoconjugate Journal","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10719-022-10073-9","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2022/7/16 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Structure and physicochemical properties of polysaccharides from Poria cocos extracted by deep eutectic solvent.
Poria cocos, a famous traditional Chinese medicine and a well-known food or food supplement, has shown therapeutic potential against cancer and the uneasiness of the mind. In addition, polysaccharides (PCPs) in this fungus were found to be various bioactive. In this work, one such PCP, PCP-1, extracted by deep eutectic solvent (DES) and separated using Sephadex G-15 columns, was characterized using GC-MS, HPGPC, FT-IR, and NMR, while also tested for physicochemical properties. Results indicated that PCP-1 contained 96.89 ± 3.21% total sugars and was a glucan with molecular weight of 3.2 kD. The main glycosidic linkage was 1,3-linked Glcp with 96.82 mol% content and a triple helix structure, and β-D-Glcp-(1 → linkage connected to the main chain through an O-6 atom was the backbone structure. In terms of the physicochemical property, PCP-1 was soluble in water, but not in organic solvent, and processed a relative high water-holding capacity (8.64 ± 0.14 g/g) and low oil-holding capacity (2.52 ± 0.21 g/g). In addition, in vitro, PCP-1 was found to have the ability of scavenging DPPH, hydroxyl free radical, superoxide anion radical and reducing ferric at different levels. This research would be useful for the further application of PCP-1.
期刊介绍:
Glycoconjugate Journal publishes articles and reviews on all areas concerned with:
function, composition, structure, biosynthesis, degradation, interactions, recognition and chemo-enzymatic synthesis of glycoconjugates (glycoproteins, glycolipids, oligosaccharides, polysaccharides and proteoglycans), biochemistry, molecular biology, biotechnology, immunology and cell biology of glycoconjugates, aspects related to disease processes (immunological, inflammatory, arthritic infections, metabolic disorders, malignancy, neurological disorders), structural and functional glycomics, glycoimmunology, glycovaccines, organic synthesis of glycoconjugates and the development of methodologies if biologically relevant, glycosylation changes in disease if focused on either the discovery of a novel disease marker or the improved understanding of some basic pathological mechanism, articles on the effects of toxicological agents (alcohol, tobacco, narcotics, environmental agents) on glycosylation, and the use of glycotherapeutics.
Glycoconjugate Journal is the official journal of the International Glycoconjugate Organization, which is responsible for organizing the biennial International Symposia on Glycoconjugates.