双氯芬酸对佛罗里达侧耳漆酶的生物转化及毒性评价。

Dhrisya Chenthamara, Sankar Ganesh Ramakrishnan, Ameer Khusro, Sadhasivam Subramaniam, Saad Alghamdi, Mazen Almehmadi, Ahmed Kabrah, Muhammad Umar Khayam Sahibzada, Aziz Eftekhari
{"title":"双氯芬酸对佛罗里达侧耳漆酶的生物转化及毒性评价。","authors":"Dhrisya Chenthamara,&nbsp;Sankar Ganesh Ramakrishnan,&nbsp;Ameer Khusro,&nbsp;Sadhasivam Subramaniam,&nbsp;Saad Alghamdi,&nbsp;Mazen Almehmadi,&nbsp;Ahmed Kabrah,&nbsp;Muhammad Umar Khayam Sahibzada,&nbsp;Aziz Eftekhari","doi":"10.14715/cmb/2021.67.5.56","DOIUrl":null,"url":null,"abstract":"<p><p>Laccase producing fungus Pleurotus floridanus was isolated from Siruvani forest, Tamil Nadu, India. The potential of P. floridanus to produce laccase by using various lignocellulosic substrates was screened under submerged fermentation. Laccase production in the presence of lignocellulosic substrates such as rice, wheat and maize bran as a sole source of carbon as well as an additional supplement was examined. Laccase activity of P. floridanus using varied substrates was observed in the order of rice bran > wheat bran > maize bran. The isolate showed maximum laccase activity of 13.29±0.01 U/mL using rice bran as a carbon source within 11 days. This was 18 fold higher than the control media that lacks lignocellulosic substrates. The diclofenac tolerance was assessed in solid media at various concentrations and the results showed that the mycelia growth is not significantly affected by the drug. Finally, the laccase mediated degradation of diclofenac at a concentration of 10 mg/L showed 98% degradation in 2 h. The phytotoxicity of the crude laccase treated diclofenac was lower than the untreated diclofenac. In conclusion, findings suggested direct application of crude laccase produced from P. floridanus using agro-residues as ideal substrate for environmental applications.</p>","PeriodicalId":520584,"journal":{"name":"Cellular and molecular biology (Noisy-le-Grand, France)","volume":" ","pages":"439-450"},"PeriodicalIF":0.0000,"publicationDate":"2022-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Diclofenac biotransformation and toxicity assessment of laccase from Pleurotus floridanus.\",\"authors\":\"Dhrisya Chenthamara,&nbsp;Sankar Ganesh Ramakrishnan,&nbsp;Ameer Khusro,&nbsp;Sadhasivam Subramaniam,&nbsp;Saad Alghamdi,&nbsp;Mazen Almehmadi,&nbsp;Ahmed Kabrah,&nbsp;Muhammad Umar Khayam Sahibzada,&nbsp;Aziz Eftekhari\",\"doi\":\"10.14715/cmb/2021.67.5.56\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Laccase producing fungus Pleurotus floridanus was isolated from Siruvani forest, Tamil Nadu, India. The potential of P. floridanus to produce laccase by using various lignocellulosic substrates was screened under submerged fermentation. Laccase production in the presence of lignocellulosic substrates such as rice, wheat and maize bran as a sole source of carbon as well as an additional supplement was examined. Laccase activity of P. floridanus using varied substrates was observed in the order of rice bran > wheat bran > maize bran. The isolate showed maximum laccase activity of 13.29±0.01 U/mL using rice bran as a carbon source within 11 days. This was 18 fold higher than the control media that lacks lignocellulosic substrates. The diclofenac tolerance was assessed in solid media at various concentrations and the results showed that the mycelia growth is not significantly affected by the drug. Finally, the laccase mediated degradation of diclofenac at a concentration of 10 mg/L showed 98% degradation in 2 h. The phytotoxicity of the crude laccase treated diclofenac was lower than the untreated diclofenac. In conclusion, findings suggested direct application of crude laccase produced from P. floridanus using agro-residues as ideal substrate for environmental applications.</p>\",\"PeriodicalId\":520584,\"journal\":{\"name\":\"Cellular and molecular biology (Noisy-le-Grand, France)\",\"volume\":\" \",\"pages\":\"439-450\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-02-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cellular and molecular biology (Noisy-le-Grand, France)\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.14715/cmb/2021.67.5.56\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellular and molecular biology (Noisy-le-Grand, France)","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.14715/cmb/2021.67.5.56","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

摘要

从印度泰米尔纳德邦Siruvani森林中分离到产漆酶真菌Pleurotus floridanus。在潜水发酵条件下,筛选了不同木质纤维素底物对floridanus产漆酶的影响。研究了木质纤维素底物(如大米、小麦和玉米麸皮)作为碳的唯一来源以及额外补充物时漆酶的产生。不同底物对褐花蓟马漆酶活性的影响顺序为米糠>麦麸>玉米糠。以米糠为碳源的分离菌在11 d内的漆酶活性最高,为13.29±0.01 U/mL。这比缺乏木质纤维素底物的对照培养基高18倍。在不同浓度的固体培养基中对双氯芬酸的耐受性进行了评估,结果表明,药物对菌丝生长没有显著影响。最后,漆酶介导的双氯芬酸在浓度为10 mg/L时的降解在2 h内达到98%。漆酶粗处理的双氯芬酸的植物毒性低于未处理的双氯芬酸。综上所述,研究结果表明,利用农业残留物作为理想的底物,直接利用由佛罗里达假单胞菌产生的粗漆酶进行环境应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diclofenac biotransformation and toxicity assessment of laccase from Pleurotus floridanus.

Laccase producing fungus Pleurotus floridanus was isolated from Siruvani forest, Tamil Nadu, India. The potential of P. floridanus to produce laccase by using various lignocellulosic substrates was screened under submerged fermentation. Laccase production in the presence of lignocellulosic substrates such as rice, wheat and maize bran as a sole source of carbon as well as an additional supplement was examined. Laccase activity of P. floridanus using varied substrates was observed in the order of rice bran > wheat bran > maize bran. The isolate showed maximum laccase activity of 13.29±0.01 U/mL using rice bran as a carbon source within 11 days. This was 18 fold higher than the control media that lacks lignocellulosic substrates. The diclofenac tolerance was assessed in solid media at various concentrations and the results showed that the mycelia growth is not significantly affected by the drug. Finally, the laccase mediated degradation of diclofenac at a concentration of 10 mg/L showed 98% degradation in 2 h. The phytotoxicity of the crude laccase treated diclofenac was lower than the untreated diclofenac. In conclusion, findings suggested direct application of crude laccase produced from P. floridanus using agro-residues as ideal substrate for environmental applications.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信