Valentin Faust , Theo A. van Alen , Huub J.M. Op den Camp , Siegfried E. Vlaeminck , Ramon Ganigué , Nico Boon , Kai M. Udert
{"title":"新型尿亚硝基酸碱候选菌对氨氧化在低pH下对工艺干扰敏感,在中性pH下对铁限制敏感","authors":"Valentin Faust , Theo A. van Alen , Huub J.M. Op den Camp , Siegfried E. Vlaeminck , Ramon Ganigué , Nico Boon , Kai M. Udert","doi":"10.1016/j.wroa.2022.100157","DOIUrl":null,"url":null,"abstract":"<div><p>Acid-tolerant ammonia-oxidizing bacteria (AOB) can open the door to new applications, such as partial nitritation at low pH. However, they can also be problematic because chemical nitrite oxidation occurs at low pH, leading to the release of harmful nitrogen oxide gases. In this publication, the role of acid-tolerant AOB in urine treatment was explored. On the one hand, the technical feasibility of ammonia oxidation under acidic conditions for source-separated urine with total nitrogen concentrations up to 3.5 g-N L<sup>−1</sup> was investigated. On the other hand, the abundance and growth of acid-tolerant AOB at more neutral pH was explored. Under acidic conditions (pH of 5), ammonia oxidation rates of 500 mg-N L<sup>−1</sup> d<sup>−1</sup> and 10 g-N g-VSS<sup>-1</sup> d<sup>-1</sup> were observed, despite high concentrations of 15 mg-N L<sup>−1</sup> of the AOB-inhibiting compound nitrous acid and low concentration of 0.04 mg-N L<sup>−1</sup> of the substrate ammonia. However, ammonia oxidation under acidic conditions was very sensitive to process disturbances. Even short periods of less than 12 h without oxygen or without influent resulted in a complete cessation of ammonia oxidation with a recovery time of up to two months, which is a problem for low maintenance applications such as decentralized treatment. Furthermore, undesirable nitrogen losses of about 10% were observed. Under acidic conditions, a novel AOB strain was enriched with a relative abundance of up to 80%, for which the name “<em>Candidatus (Ca.)</em> Nitrosacidococcus urinae” is proposed. While <em>Nitrosacidococcus</em> members were present only to a small extent (0.004%) in urine nitrification reactors operated at pH values between 5.8 and 7, acid-tolerant AOB were always enriched during long periods without influent, resulting in an uncontrolled drop in pH to as low as 2.5. Long-term experiments at different pH values showed that the activity of “<em>Ca.</em> Nitrosacidococcus urinae” decreased strongly at a pH of 7, where they were also outcompeted by the acid-sensitive AOB <em>Nitrosomonas halophila</em>. The experiment results showed that the decreased activity of “<em>Ca.</em> Nitrosacidococcus urinae” correlated with the limited availability of dissolved iron at neutral pH.</p></div>","PeriodicalId":52198,"journal":{"name":"Water Research X","volume":null,"pages":null},"PeriodicalIF":7.2000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574496/pdf/","citationCount":"5","resultStr":"{\"title\":\"Ammonia oxidation by novel “Candidatus Nitrosacidococcus urinae” is sensitive to process disturbances at low pH and to iron limitation at neutral pH\",\"authors\":\"Valentin Faust , Theo A. van Alen , Huub J.M. Op den Camp , Siegfried E. Vlaeminck , Ramon Ganigué , Nico Boon , Kai M. Udert\",\"doi\":\"10.1016/j.wroa.2022.100157\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Acid-tolerant ammonia-oxidizing bacteria (AOB) can open the door to new applications, such as partial nitritation at low pH. However, they can also be problematic because chemical nitrite oxidation occurs at low pH, leading to the release of harmful nitrogen oxide gases. In this publication, the role of acid-tolerant AOB in urine treatment was explored. On the one hand, the technical feasibility of ammonia oxidation under acidic conditions for source-separated urine with total nitrogen concentrations up to 3.5 g-N L<sup>−1</sup> was investigated. On the other hand, the abundance and growth of acid-tolerant AOB at more neutral pH was explored. Under acidic conditions (pH of 5), ammonia oxidation rates of 500 mg-N L<sup>−1</sup> d<sup>−1</sup> and 10 g-N g-VSS<sup>-1</sup> d<sup>-1</sup> were observed, despite high concentrations of 15 mg-N L<sup>−1</sup> of the AOB-inhibiting compound nitrous acid and low concentration of 0.04 mg-N L<sup>−1</sup> of the substrate ammonia. However, ammonia oxidation under acidic conditions was very sensitive to process disturbances. Even short periods of less than 12 h without oxygen or without influent resulted in a complete cessation of ammonia oxidation with a recovery time of up to two months, which is a problem for low maintenance applications such as decentralized treatment. Furthermore, undesirable nitrogen losses of about 10% were observed. Under acidic conditions, a novel AOB strain was enriched with a relative abundance of up to 80%, for which the name “<em>Candidatus (Ca.)</em> Nitrosacidococcus urinae” is proposed. While <em>Nitrosacidococcus</em> members were present only to a small extent (0.004%) in urine nitrification reactors operated at pH values between 5.8 and 7, acid-tolerant AOB were always enriched during long periods without influent, resulting in an uncontrolled drop in pH to as low as 2.5. Long-term experiments at different pH values showed that the activity of “<em>Ca.</em> Nitrosacidococcus urinae” decreased strongly at a pH of 7, where they were also outcompeted by the acid-sensitive AOB <em>Nitrosomonas halophila</em>. The experiment results showed that the decreased activity of “<em>Ca.</em> Nitrosacidococcus urinae” correlated with the limited availability of dissolved iron at neutral pH.</p></div>\",\"PeriodicalId\":52198,\"journal\":{\"name\":\"Water Research X\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":7.2000,\"publicationDate\":\"2022-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9574496/pdf/\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Water Research X\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2589914722000275\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water Research X","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589914722000275","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Ammonia oxidation by novel “Candidatus Nitrosacidococcus urinae” is sensitive to process disturbances at low pH and to iron limitation at neutral pH
Acid-tolerant ammonia-oxidizing bacteria (AOB) can open the door to new applications, such as partial nitritation at low pH. However, they can also be problematic because chemical nitrite oxidation occurs at low pH, leading to the release of harmful nitrogen oxide gases. In this publication, the role of acid-tolerant AOB in urine treatment was explored. On the one hand, the technical feasibility of ammonia oxidation under acidic conditions for source-separated urine with total nitrogen concentrations up to 3.5 g-N L−1 was investigated. On the other hand, the abundance and growth of acid-tolerant AOB at more neutral pH was explored. Under acidic conditions (pH of 5), ammonia oxidation rates of 500 mg-N L−1 d−1 and 10 g-N g-VSS-1 d-1 were observed, despite high concentrations of 15 mg-N L−1 of the AOB-inhibiting compound nitrous acid and low concentration of 0.04 mg-N L−1 of the substrate ammonia. However, ammonia oxidation under acidic conditions was very sensitive to process disturbances. Even short periods of less than 12 h without oxygen or without influent resulted in a complete cessation of ammonia oxidation with a recovery time of up to two months, which is a problem for low maintenance applications such as decentralized treatment. Furthermore, undesirable nitrogen losses of about 10% were observed. Under acidic conditions, a novel AOB strain was enriched with a relative abundance of up to 80%, for which the name “Candidatus (Ca.) Nitrosacidococcus urinae” is proposed. While Nitrosacidococcus members were present only to a small extent (0.004%) in urine nitrification reactors operated at pH values between 5.8 and 7, acid-tolerant AOB were always enriched during long periods without influent, resulting in an uncontrolled drop in pH to as low as 2.5. Long-term experiments at different pH values showed that the activity of “Ca. Nitrosacidococcus urinae” decreased strongly at a pH of 7, where they were also outcompeted by the acid-sensitive AOB Nitrosomonas halophila. The experiment results showed that the decreased activity of “Ca. Nitrosacidococcus urinae” correlated with the limited availability of dissolved iron at neutral pH.
Water Research XEnvironmental Science-Water Science and Technology
CiteScore
12.30
自引率
1.30%
发文量
19
期刊介绍:
Water Research X is a sister journal of Water Research, which follows a Gold Open Access model. It focuses on publishing concise, letter-style research papers, visionary perspectives and editorials, as well as mini-reviews on emerging topics. The Journal invites contributions from researchers worldwide on various aspects of the science and technology related to the human impact on the water cycle, water quality, and its global management.