预处理强化训练可改善脑缺血后轻瘫肌pgc1 α-通路转录生物因子的减少。

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnic & Histochemistry Pub Date : 2023-01-01 Epub Date: 2022-07-27 DOI:10.1080/10520295.2022.2098535
Sadegh Abbasian, Ali Asghar Ravasi, Amir Hossein Haghighi, Suna Aydin, Ahmad Delbari, Suleyman Aydın
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引用次数: 0

摘要

运动训练通过过氧化物酶体增殖物激活受体γ辅助激活因子1- α (PGC1α)途径增加纤维连接蛋白III型结构域含蛋白5 (FNDC5/鸢尾素)。运动训练和缺血性中风后,PGC1α通路诱导FNDC5/鸢尾素的变化尚不完全清楚。研究PGC-1α/FNDC5/鸢尾素通路与运动训练及缺血性脑卒中模型大鼠麻痹肌病理生理的关系。我们在完成高强度间歇训练(HIIT)后诱导脑缺血,以评估paretic肌肉中的pgc1 α-通路生物因子。为了明确脑缺血后麻痹性肌肉改善的潜在分子机制,我们使用免疫荧光跟踪和酶联免疫吸附试验(ELISA)免疫分析法评估了pcg -1α-途径因子。我们发现,HIIT 3周后,中风大鼠血清和麻痹肌中pgc -1α-通路生物标志物的表达和释放均增加。我们还发现骨骼肌中pcg -1α-通路因子的表达与血中pcg -1α-通路因子的浓度密切相关。我们发现pgc -1α-通路生物标志物在脑缺血诱导后引起鸢尾素上调。pgc -1α-通路生物标志物增加后神经功能缺陷的减少表明,这些因素可能是脑缺血后麻痹性肌肉愈合改善的标志。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preconditioning intensive training ameliorates reduction of transcription biofactors of PGC1α-pathway in paretic muscle due to cerebral ischemia.

Exercise training increases fibronectin type III domain-containing protein 5 (FNDC5/irisin) via the peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α)-pathway. The PGC1α pathway induced FNDC5/irisin changes in response to exercise training and ischemic stroke are not entirely understood. We investigated the relation of the PGC-1α/FNDC5/irisin pathway to exercise training and to the pathophysiology of ischemic stroke in paretic muscles of stroke-induced rat models. We induced cerebral ischemia following completion of high-intensity interval training (HIIT) to evaluate PGC1α-pathway biofactors in paretic muscles. To define the underlying molecular mechanisms for improvement in paretic muscles following cerebral ischemia, we evaluated PCG-1α-pathway factors using immunofluorescence tracking and enzyme-linked immunosorbent assay (ELISA) immunoassay. We found that HIIT for 3 weeks produced increased expression and release of PGC-1α-pathway biomarkers in both the serum and paretic muscle of stroke-induced rats. We also found a close relation between the expression of PCG-1α-pathway factors in skeletal muscle and their concentration in blood. We found that PGC-1α-pathway biomarkers cause irisin up-regulation following induction of cerebral ischemia. The reduction in neurofunctional deficits following increased PGC-1α-pathway biomarkers suggests that these factors may act as markers of improvement in paretic muscle healing following cerebral ischemia.

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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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