人类卵巢冷冻保存:从组织学到基因表达的玻璃化与缓慢冷冻。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2023-12-01 Epub Date: 2022-11-17 DOI:10.1080/14647273.2022.2136540
Pauline Jaeger, Cyrielle Fournier, Claire Santamaria, Eloise Fraison, Nicolas Morel-Journel, Mehdi Benchaib, Bruno Salle, Jacqueline Lornage, Elsa Labrune
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引用次数: 0

摘要

卵巢组织冷冻保存是为需要接受性腺毒性治疗的女孩和妇女提供的保留生育能力的策略之一。冷冻保存的参考方法是缓慢冷冻,玻璃化是另一种方法。我们的目的是评估这两种方法中哪一种是人类卵巢组织冷冻保存的最佳方法。每个卵巢被分为三组:(i) 新鲜;(ii) 缓慢冷冻;(iii) 玻璃化。对卵泡密度、质量和六种基因(CYP11A、STAR、GDF9、ZP3、CDK2、CDKN1A)的表达进行了评估。我们观察到这三个组的卵泡密度没有明显差异。与新鲜组织相比,缓慢冷冻改变了原始卵泡(31.8% vs 55.9%,p = 0.046)。与新鲜组相比,低温冷冻后参与类固醇生成的基因表达有所变化;CYP11A在低温冷冻组表达不足(p = 0.01),STAR在玻璃化组表达不足(p = 0.01)。在参与细胞周期调控的基因表达方面,CDKN1A在冷冻组和玻璃化组都明显表达不足(缓慢冷冻组:p = 0.0008;玻璃化组:p = 0.03)。与新鲜组织相比,玻璃化对卵泡任何发育阶段的组织学质量都没有影响。两种技术在基因表达方面没有明显差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Human ovarian cryopreservation: vitrification versus slow freezing from histology to gene expression.

Cryopreservation of ovarian tissue is one of the strategies offered to girls and women needing gonadotoxic treatment to preserve their fertility. The reference method to cryopreserve is slow freezing; vitrification is an alternative method. The aim was to evaluate which of the two is the best method for human ovarian tissue cryopreservation. Each ovary was divided into three groups: (i) fresh; (ii) slow freezing; and (iii) vitrification. An evaluation of the follicular density, quality and the expression six genes (CYP11A, STAR, GDF9, ZP3, CDK2, CDKN1A) were performed. We observed no significant difference in follicular density within these three groups. Slow freezing altered the primordial follicles compared to the fresh tissue (31.8% vs 55.9%, p = 0.046). The expression of genes involved in steroidogenesis varied after cryopreservation compared to the fresh group; CYP11A was under-expressed in slow freezing group (p = 0.01), STAR was under-expressed in the vitrification group (p = 0.01). Regarding the expression of genes involved in cell cycle regulation, CDKN1A was significantly under-expressed in both freezing groups (slow freezing: p = 0.0008; vitrification: p = 0.03). Vitrification had no effect on the histological quality of the follicles at any stage of development compared to fresh tissue. There was no significant difference in gene expression between the two techniques.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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