{"title":"TiO2纳米颗粒的合成、表征及在水牛膝草(Bacopa monnieri L. Pennel)再生中的应用","authors":"Muhammad Aasim, Ecenur Korkmaz, Ayse Culu, Burak Kahveci, Ozlem Ates Sonmezoglu","doi":"10.1080/10520295.2022.2087236","DOIUrl":null,"url":null,"abstract":"<p><p>Water hyssop (<i>Bacopa monnieri</i> L. Pennel) is a medicinal aquatic herb used to treat diseases in South Asia. Various regeneration protocols have been developed or modified in vitro to ensure the availability of biomass and secondary metabolites of <i>Bacopa</i>. We applied hydrothermally treated titanium dioxide (TiO<sub>2</sub>) nanoparticles (NPs) (TiO<sub>2</sub>-NPs) at different concentrations. Three explants, distal portion of half leaf (DPHL), proximal portion of half leaf (PPHL) and full leaf (FL), were used to evaluate response to TiO<sub>2</sub>. Regeneration from the three explants in vitro was similar except for shoot length. Application of TiO<sub>2</sub>-NPs exerted significant, but variable, effects on all parameters except percentage of shoot formation, which was 100%. Interactive effects of explant and TiO<sub>2</sub>-NPs exhibited significant, but variable, effects on fresh weight and percentage of callus formation. All explants produced more shoots using TiO<sub>2</sub>-NPs compared to control treatments. DPHL explants with application of 8 mg/l TiO<sub>2</sub> produced more shoots than controls. Similarly, FL explant treated with 2 mg/l TiO<sub>2</sub>-NPs produced more shoots/explant than controls. All concentrations of TiO<sub>2</sub>-NPs produced significantly longer shoots compared to controls. Increased elongation of shoots justifies use of TiO<sub>2</sub>-NPs for propagation of plants in vitro during acclimatization. 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DPHL explants with application of 8 mg/l TiO<sub>2</sub> produced more shoots than controls. Similarly, FL explant treated with 2 mg/l TiO<sub>2</sub>-NPs produced more shoots/explant than controls. All concentrations of TiO<sub>2</sub>-NPs produced significantly longer shoots compared to controls. Increased elongation of shoots justifies use of TiO<sub>2</sub>-NPs for propagation of plants in vitro during acclimatization. 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引用次数: 2
摘要
水牛膝草(Bacopa monnieri L. Pennel)是一种用于治疗南亚疾病的药用水生草药。为了保证假马齿苋生物量和次生代谢产物的有效性，已经开发或修改了各种体外再生方案。我们应用不同浓度的水热处理二氧化钛纳米粒子(TiO2-NPs)。采用半叶远端部分(DPHL)、半叶近端部分(PPHL)和全叶(FL)三个外植体对TiO2的反应进行评价。三种外植体的离体再生除了茎长不同外，其余均相似。TiO2-NPs的施用对除新梢形成率(100%)外的所有参数均有显著但可变的影响。外植体与TiO2-NPs的交互作用对愈伤组织的鲜重和成活率有显著的影响。与对照处理相比，使用TiO2-NPs处理的外植体均能产生更多的芽。TiO2浓度为8 mg/l的DPHL外植体的芽数高于对照。同样，2 mg/l TiO2-NPs处理的FL外植体每个外植体的芽数也比对照多。与对照相比，所有浓度的TiO2-NPs均产生较长的芽。芽伸长的增加证明了在驯化过程中使用TiO2-NPs进行植物离体繁殖是合理的。利用TiO2-NPs促进芽的快速伸长最终促进植物的存活。
TiO2 nanoparticle synthesis, characterization and application to shoot regeneration of water hyssop (Bacopa monnieri L. Pennel) in vitro.
Water hyssop (Bacopa monnieri L. Pennel) is a medicinal aquatic herb used to treat diseases in South Asia. Various regeneration protocols have been developed or modified in vitro to ensure the availability of biomass and secondary metabolites of Bacopa. We applied hydrothermally treated titanium dioxide (TiO2) nanoparticles (NPs) (TiO2-NPs) at different concentrations. Three explants, distal portion of half leaf (DPHL), proximal portion of half leaf (PPHL) and full leaf (FL), were used to evaluate response to TiO2. Regeneration from the three explants in vitro was similar except for shoot length. Application of TiO2-NPs exerted significant, but variable, effects on all parameters except percentage of shoot formation, which was 100%. Interactive effects of explant and TiO2-NPs exhibited significant, but variable, effects on fresh weight and percentage of callus formation. All explants produced more shoots using TiO2-NPs compared to control treatments. DPHL explants with application of 8 mg/l TiO2 produced more shoots than controls. Similarly, FL explant treated with 2 mg/l TiO2-NPs produced more shoots/explant than controls. All concentrations of TiO2-NPs produced significantly longer shoots compared to controls. Increased elongation of shoots justifies use of TiO2-NPs for propagation of plants in vitro during acclimatization. Use of TiO2-NPs for rapid elongation of shoots ultimately fosters survival of plants.
期刊介绍：
Biotechnic & Histochemistry (formerly Stain technology) is the
official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926.
Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well.
Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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