LL-37类似肽对念珠菌体外抑菌活性的研究。

Gladys Pinilla, Yenifer Tatiana Coronado, Gabriel Chaves, Liliana Muñoz, Jeannette Navarrete, Luz Mary Salazar, Carlos Pelleschi Taborda, Julián E Muñoz
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引用次数: 4

摘要

近几十年来,真菌感染增加,发病率和死亡率相当高,主要发生在免疫抑制或icu住院患者中。真菌对常规抗真菌治疗的耐药性已成为一个公共卫生问题,特别是念珠菌对几种抗真菌药物产生耐药性。因此,开发新的抗真菌疗法是至关重要的。其中一种可能性是使用抗菌肽,如LL-37,其作用于微生物膜的破坏并促进宿主的免疫调节作用。在这项研究中,我们评估了LL-37类似肽(AC-1, LL37-1, AC-2和D)对不同假丝酵母菌的体外抗真菌活性,以及从外阴阴道假丝酵母菌病患者获得的临床分离株。结果表明,对所研究菌株mic的最佳范围为LL37-1和AC-2(0.07µM)。通过分析酵母生长曲线证实了这种抑制作用,表明暴露于LL-37肽后真菌生长显著减少。通过XTT技术,我们观察到与未经类似肽处理的酵母相比,生物膜形成过程显着减少。综上所述,LL-37类似肽可能对假丝酵母具有重要的抑菌作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

In Vitro Antifungal Activity of LL-37 Analogue Peptides against <i>Candida</i> spp.

In Vitro Antifungal Activity of LL-37 Analogue Peptides against <i>Candida</i> spp.

In Vitro Antifungal Activity of LL-37 Analogue Peptides against <i>Candida</i> spp.

In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida spp.

Fungal infections have increased in recent decades with considerable morbidity and mortality, mainly in immunosuppressed or admitted-to-the-ICU patients. The fungal resistance to conventional antifungal treatments has become a public health problem, especially with Candida that presents resistance to several antifungals. Therefore, generating new alternatives of antifungal therapy is fundamental. One of these possibilities is the use of antimicrobial peptides, such as LL-37, which acts on the disruption of the microorganism membrane and promotes immunomodulatory effects in the host. In this study, we evaluated the in vitro antifungal activity of the LL-37 analogue peptides (AC-1, LL37-1, AC-2, and D) against different Candida spp. and clinical isolates obtained from patients with vulvovaginal candidiasis. Our results suggest that the peptides with the best ranges of MICs were LL37-1 and AC-2 (0.07 µM) against the strains studied. This inhibitory effect was confirmed by analyzing the yeast growth curves that evidenced a significant decrease in the fungal growth after exposure to LL-37 peptides. By the XTT technique we observed a significant reduction in the biofilm formation process when compared to yeasts untreated with the analogue peptides. In conclusion, we suggest that LL-37 analogue peptides may play an important antimicrobial role against Candida spp.

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