Wistar大鼠第一次生精波中凋亡出现及SYCP3蛋白分布改变的组织化学研究。

IF 2.1
Anatomical record (Hoboken, N.J. : 2007) Pub Date : 2019-11-01 Epub Date: 2019-06-18 DOI:10.1002/ar.24187
María L Escobar, Olga M Echeverría, Yunuen M Valenzuela, Rosario Ortiz, Nayeli Torres-Ramírez, Gerardo H Vázquez-Nin
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引用次数: 5

摘要

细胞凋亡是一种负责维持组织稳态的细胞死亡类型,可发生在男性性腺中。凋亡的形态学和生化特征包括细胞收缩、半胱天冬酶活化和DNA断裂。细胞更新和分化的动态过程发生在精小管内部,它们分别受有丝分裂和减数分裂的调控。在减数分裂过程中,重组是由突触复合体的组装引起的,这涉及到组成蛋白的参与,如突触复合体蛋白-3 (SYCP3)。本研究评估了未成熟雄性大鼠的生发细胞死亡和SYCP3蛋白的分布。我们的研究结果表明,随着生发细胞进入第二个减数分裂阶段,大量的细胞被凋亡消除。我们确定SYCP3蛋白并不总是结合到突触复合体的结构中,而是在核膜附近形成核积云,导致许多这些细胞发生凋亡。我们认为SYCP3蛋白的过量及其在第一次减数分裂过程中的积累可能导致了青春期前Wistar大鼠第一次生精波中原代精母细胞的细胞死亡。中国生物医学工程学报,2016,32(2):882 - 892。©2019美国解剖学协会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Histochemical Study of the Emergence of Apoptosis and Altered SYCP3 Protein Distribution During the First Spermatogenic Wave in Wistar Rats.

Apoptosis is a type of cell death responsible for maintaining tissue homeostasis that can occur in male gonads. The morphological and biochemical characteristics of apoptosis include cellular contraction, caspase activation, and DNA fragmentation. Dynamic processes of cell renewal and differentiation occur inside the seminiferous tubules, which are regulated by mitosis and meiosis, respectively. During meiosis, recombination is caused by assembly of the synaptonemal complex, which involves the participation of constitutive proteins, such as synaptonemal complex protein-3 (SYCP3). The present study evaluated germinal cell death in immature male rats and the distribution of the SYCP3 protein. Our results indicate that as germinal cells progress to the second meiotic stage, significant numbers of them are eliminated by apoptosis. We determined that the SYCP3 protein is not always incorporated into the structure of the synaptonemal complex but rather forms a nuclear cumulus near the inner nuclear membrane, causing many of these cells to undergo apoptosis. We propose that both the excess of the SYCP3 protein and its accumulation during the first meiotic division could contribute to the cell death of primary spermatocytes during the first spermatogenic wave in prepubertal Wistar rats. Anat Rec, 302:2082-2092, 2019. © 2019 American Association for Anatomy.

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