Kyryl Zagorovsky, Maria Teresa Fernández-Argüelles, Diane Bona, Ashraf Mohamed Elshawadfy, Abdullah Muhammad Syed, Pranav Kadhiresan, Tony Mazzulli, Karen L. Maxwell and Warren C.W. Chan*,
{"title":"诊断尿路感染的金纳米粒子智能手机平台","authors":"Kyryl Zagorovsky, Maria Teresa Fernández-Argüelles, Diane Bona, Ashraf Mohamed Elshawadfy, Abdullah Muhammad Syed, Pranav Kadhiresan, Tony Mazzulli, Karen L. Maxwell and Warren C.W. Chan*, ","doi":"10.1021/acsnanoscienceau.2c00001","DOIUrl":null,"url":null,"abstract":"<p >Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 10<sup>5</sup> bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with <i>Escherichia coli</i>, <i>Proteus mirabilis,</i> and <i>Klebsiella pneumoniae</i> infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51–73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.</p>","PeriodicalId":29799,"journal":{"name":"ACS Nanoscience Au","volume":"2 4","pages":"324–332"},"PeriodicalIF":4.8000,"publicationDate":"2022-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9a/b0/ng2c00001.PMC9389610.pdf","citationCount":"6","resultStr":"{\"title\":\"Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections\",\"authors\":\"Kyryl Zagorovsky, Maria Teresa Fernández-Argüelles, Diane Bona, Ashraf Mohamed Elshawadfy, Abdullah Muhammad Syed, Pranav Kadhiresan, Tony Mazzulli, Karen L. Maxwell and Warren C.W. Chan*, \",\"doi\":\"10.1021/acsnanoscienceau.2c00001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 10<sup>5</sup> bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with <i>Escherichia coli</i>, <i>Proteus mirabilis,</i> and <i>Klebsiella pneumoniae</i> infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51–73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.</p>\",\"PeriodicalId\":29799,\"journal\":{\"name\":\"ACS Nanoscience Au\",\"volume\":\"2 4\",\"pages\":\"324–332\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2022-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9a/b0/ng2c00001.PMC9389610.pdf\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Nanoscience Au\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://pubs.acs.org/doi/10.1021/acsnanoscienceau.2c00001\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NANOSCIENCE & NANOTECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Nanoscience Au","FirstCategoryId":"1085","ListUrlMain":"https://pubs.acs.org/doi/10.1021/acsnanoscienceau.2c00001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NANOSCIENCE & NANOTECHNOLOGY","Score":null,"Total":0}
Gold Nanoparticle Smartphone Platform for Diagnosing Urinary Tract Infections
Current urinary tract infection (UTI) diagnostic methods are slow or provide limited information, resulting in prescribing antibiotic therapy before bacterial pathogen identification. Here, we adapted a gold nanoparticle colorimetric approach and developed a smartphone platform for UTI detection. We show the parallel identification of five major UTI pathogens at clinically relevant concentrations of 105 bacteria/mL using bacteria-specific and universal probes. We validated the diagnostic technology using 115 positive and 19 negative samples from patients with Escherichia coli, Proteus mirabilis, and Klebsiella pneumoniae infections. The assay successfully identified the infecting pathogen (specificity: >98% and sensitivity: 51–73%) in 3 h. Our platform is faster than culturing and can wirelessly store and transmit results at the cost of $0.38 per assay.
期刊介绍：
ACS Nanoscience Au is an open access journal that publishes original fundamental and applied research on nanoscience and nanotechnology research at the interfaces of chemistry biology medicine materials science physics and engineering.The journal publishes short letters comprehensive articles reviews and perspectives on all aspects of nanoscience and nanotechnology:synthesis assembly characterization theory modeling and simulation of nanostructures nanomaterials and nanoscale devicesdesign fabrication and applications of organic inorganic polymer hybrid and biological nanostructuresexperimental and theoretical studies of nanoscale chemical physical and biological phenomenamethods and tools for nanoscience and nanotechnologyself- and directed-assemblyzero- one- and two-dimensional materialsnanostructures and nano-engineered devices with advanced performancenanobiotechnologynanomedicine and nanotoxicologyACS Nanoscience Au also publishes original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials engineering physics bioscience and chemistry into important applications of nanomaterials.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
