Haiqi Fu, Xinhao Yang, Rong Hao, Xiuli Han, Shu Song, Yan Guo, Yongqing Yang
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引用次数: 0
摘要
拟南芥中的气孔关闭相关肌动蛋白结合蛋白 1（SCAB1）通过介导肌动蛋白丝重组来调节气孔关闭。我们之前的研究表明，3-磷酸肌醇磷脂（PI3P）能与 SCAB1 结合并抑制其寡聚化，从而抑制其在气孔关闭过程中对防护细胞中 F-肌动蛋白的活性。在本研究中，我们发现另一种磷脂--磷脂酸（PA）也能与 SCAB1 结合，并抑制其肌动蛋白束缚活性，但不抑制其肌动蛋白结合活性。用抑制 PA 生成的正丁醇处理 Col-0 幼苗可促进体内肌动蛋白的捆绑，但在 scab1 突变体中却没有这种效果。这些结果表明，信号分子 PA 参与了 SCAB1 在 F-肌动蛋白重组中的活性调节。
Phosphatidic acid inhibits SCAB1-mediated F-actin bundling in Arabidopsis.
Stomatal closure-associated actin-binding protein 1 (SCAB1) regulates stomatal closure by mediating actin filament reorganization in Arabidopsis thaliana. Our previous study showed that phosphatidylinositol 3-phosphate (PI3P) binds to SCAB1 and inhibits its oligomerization, thereby inhibiting its activity on F-actin in guard cells during stomatal closure. In this study, we show that another phospholipid, phosphatidic acid (PA), also binds to SCAB1 and inhibits its actin-bundling activity but not its actin-binding activity. F-actin bundling was promoted in vivo by treating Col-0 seedlings with n-butanol, a suppressor of PA production, but this effect was absent in the scab1 mutant. These results indicate that the signaling molecule PA is involved in the modulation of SCAB1 activity in F-actin reorganization.
期刊介绍：
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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