Haiqi Fu, Xinhao Yang, Rong Hao, Xiuli Han, Shu Song, Yan Guo, Yongqing Yang
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引用次数: 0
摘要
拟南芥中的气孔关闭相关肌动蛋白结合蛋白 1（SCAB1）通过介导肌动蛋白丝重组来调节气孔关闭。我们之前的研究表明，3-磷酸肌醇磷脂（PI3P）能与 SCAB1 结合并抑制其寡聚化，从而抑制其在气孔关闭过程中对防护细胞中 F-肌动蛋白的活性。在本研究中，我们发现另一种磷脂--磷脂酸（PA）也能与 SCAB1 结合，并抑制其肌动蛋白束缚活性，但不抑制其肌动蛋白结合活性。用抑制 PA 生成的正丁醇处理 Col-0 幼苗可促进体内肌动蛋白的捆绑，但在 scab1 突变体中却没有这种效果。这些结果表明，信号分子 PA 参与了 SCAB1 在 F-肌动蛋白重组中的活性调节。
Phosphatidic acid inhibits SCAB1-mediated F-actin bundling in Arabidopsis.
Stomatal closure-associated actin-binding protein 1 (SCAB1) regulates stomatal closure by mediating actin filament reorganization in Arabidopsis thaliana. Our previous study showed that phosphatidylinositol 3-phosphate (PI3P) binds to SCAB1 and inhibits its oligomerization, thereby inhibiting its activity on F-actin in guard cells during stomatal closure. In this study, we show that another phospholipid, phosphatidic acid (PA), also binds to SCAB1 and inhibits its actin-bundling activity but not its actin-binding activity. F-actin bundling was promoted in vivo by treating Col-0 seedlings with n-butanol, a suppressor of PA production, but this effect was absent in the scab1 mutant. These results indicate that the signaling molecule PA is involved in the modulation of SCAB1 activity in F-actin reorganization.
期刊介绍：
Plant Signaling & Behavior, a multidisciplinary peer-reviewed journal published monthly online, publishes original research articles and reviews covering the latest aspects of signal perception and transduction, integrative plant physiology, and information acquisition and processing.
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